Development of modified tympanoplasty combined with transplantation of artificial middle ear mucosa and drug delivery system

改良鼓室成形术联合人工中耳粘膜移植及给药系统的研制

• 批准号: 19591990
• 负责人: KOJIMA Hiromi
• 金额: $ 2.08万
• 依托单位: Jikei University School of Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2007
• 资助国家: 日本
• 起止时间: 2007 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-19591990/
• 关键词: 中耳粘膜; 再生; DDS; 鼓室形成術; 中耳炎; 培養

We have developed successfully middle ear mucosal epithelial cell sheets with three-dimensional structure, analogous to normal middle ear mucosa. Furthermore, we found it clear that a rapid mucosal regeneration is boosted by transplanting cell sheet created with autologous cells on to the rabbit's middle ear mucosa missing area. In addition, autologous human nasal epithelial cell sheet using temperature-responsive culture insert was made successfully. By histologically studying artificial human nasal epithelial cell sheets, we confirmed the presence of epidermal cell-like cells in the cortex and fibroblast-like cells in the substratum. The sheets consisted of multi-layered mucosal epithelia and lower connective tissue and were similar to normal middle ear mucosa. Scanning electron microscopy revealed a cobblestone-like pattern of cells with innumerable microvilli and a cilium-like configuration. We also confirmed the basal membrane configuration that included tight junctions, desmosomes, and cell adhesion structures among the epidermal layers.. This artificial mucosa had a similar configuration to that of normal middle ear mucosa.For these reasons, middle ear mucosal cell sheet is considered as an effective transplantation material for mucosal regeneration in otitis media surgery, and it is expected to apply for human clinical use in the future.

我们已经成功地开发了中耳粘膜上皮细胞片的三维结构，类似于正常中耳粘膜。此外，我们清楚地发现，通过将用自体细胞产生的细胞片移植到兔的中耳粘膜缺失区域上，促进了快速的粘膜再生。此外，利用温度响应性培养插入物成功制备了自体人鼻上皮细胞片层。通过组织学研究人工人鼻上皮细胞片，我们证实了表皮细胞样细胞在皮质和成纤维细胞样细胞在基底层的存在。该片由多层粘膜上皮和下层结缔组织组成，与正常中耳粘膜相似。扫描电子显微镜显示鹅卵石样图案的细胞与无数的微绒毛和纤毛样配置。我们还证实了基底膜的配置，包括紧密连接，桥粒，和表皮层之间的细胞粘附结构。该人工黏膜具有与正常中耳黏膜相似的形态结构，是一种有效的中耳黏膜再生移植材料，有望在临床上应用。

项目成果

Middle ear regenereration using transplantation of tissue-engineered cell sheet.

使用组织工程细胞片移植进行中耳再生。

• 发表时间: 2010
• 作者: 濱孝憲,小島博己;ほか;小島博己;小島博己;小島博己
• 通讯作者: 小島博己

中耳粘膜再生に向けて-粘膜上皮細胞シートによる検討-～シンポジウム～

迈向中耳粘膜再生 - 使用粘膜上皮细胞片的研究 - 研讨会

• 发表时间: 2009
• 作者: 山本和央;内水浩貴;谷口雄一郎;田中康広;小島博己;森山寛;小島博己
• 通讯作者: 小島博己

中耳真珠腫の手術適応と手術法について

关于中耳胆脂瘤的手术指征和手术方法

• 发表时间: 2009
• 期刊: 都耳鼻会報 130
• 作者: 田中康広,小島博己;ほか;小島博己
• 通讯作者: 小島博己

Middle ear mucosa regeneration by grafting of three-demensional middle ear mucosa organ.

三维中耳粘膜器官移植的中耳粘膜再生。

• 发表时间: 2008
• 期刊: Mediterranean Journal of Otology 4
• 作者: Yamamoto K;Hiromi Kojima;et al.
• 通讯作者: et al.

Middle ear regeneration using transplantationof tissue-engineered cell sheet

利用组织工程细胞片移植进行中耳再生

• 发表时间: 2012
• 作者: Ｙａｓｕｙａ Ｎｏｍｕｒａ;Ｈｉｔｏｍｅ Ｋｏｂａｙａｓｈｉ;比野平恭之;Hiromi Kojima;Yasuya Nomura;小島博己;小林一女;比野平恭之;小島博己;Hitome Kobayashi;Hiromi Kojima
• 通讯作者: Hiromi Kojima