Global gene expression in periodontopathogeic bacterium biofilm

牙周致病细菌生物膜中的整体基因表达

• 批准号: 19592127
• 负责人: KAMAGUCHI Arihide
• 金额: $ 3万
• 依托单位: Health Sciences University of Hokkaido
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2007
• 资助国家: 日本
• 起止时间: 2007 至 2009
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-19592127/
• 关键词: Porphyromonas gingivalis; バイオフィルム; 浮遊菌; DNAアレイ; クオーラムセンシング; AI-2; 菌の遊離; Streptococcus gordonii; 口腔細菌; 歯周病; 歯周病原因菌; タンパク質; Porphytomonas gingivalis; 遺伝子; 遺伝子発現

Gene expression analysis between biofilm cells and planktonic cells of Porphyromonas gingivalis which is one of the major aetiological agents in advance adult periodontitis was done with DNA microarray. Upregulated genes were gene for uptake of nutrient and secretion of signal molecules, gene for energy metabolism, gene for two component system and so on. Downregulated genes were gene for heat shock protein, gene for transcription under oxidative stress, phosphatase and so on. In this study, it was shown that difference of gene expression occurred between biofilm cells and plaktonic cells of P. gingivalis. Besides, it implies that that ABC trasporters are associated to formation of P. gingivalis biofilm.

应用基因芯片技术对晚期成人牙周炎的主要病原菌之一牙龈卟啉单胞菌生物膜细胞和牙周膜细胞的基因表达进行了分析。结果表明，牙龈卟啉单胞菌生物膜细胞和斑块细胞的基因表达存在差异，上调的基因有营养摄取和信号分子分泌基因、能量代谢基因、双组分系统基因等，下调的基因有热休克蛋白基因、氧化应激转录基因、磷酸酶基因等。此外，这意味着ABC转运蛋白与牙龈卟啉单胞菌生物膜的形成有关。