Participation mechanism of periodontopathogenic bacteria into biofilm composed from subgingival bacteria flora with early colonizer

共聚集是口腔细菌的定植机制之一。观察到牙龈卟啉单胞菌与中间普氏菌聚集，中间普氏菌不仅存在于牙周袋中，也存在于正常牙龈缝中。从牙龈假单胞菌囊泡中纯化了一些推测的共聚集因子。从牙龈假单胞菌ATCC 33277染色体DNA中克隆了其中一个共聚集因子基因。该共聚集因子基因序列与rgp A基因的HGP 17结构域基因具有同源性。gst - hgp17蛋白的融合蛋白溶液不聚集中间芽孢杆菌ATCC 25611。然而，P.intermedia ATCC 25611粘附在与GST-HGP 17蛋白结合的谷胱甘肽Sepharose 4B头上。P.intermedia ATCC25611粘附于谷胱甘肽Sepharose 4B头，分别与GST-HGP44、GST-HGP15、GST-HGP27结合。HGP44、HGP17、HGP27、HGP15氨基酸序列具有同源性。P.intermedia ATCC 25611粘附于谷胱甘肽Sepharose 4B头，该头结合了HGP44内部与HGP17和27同源的区域GST-HGP44F和HGP44内部与HGP15同源的区域GST-HGP44R。抗HGP 44单克隆抗体仅弱抑制p .中间体ATCC 25611的聚集，而p .牙龈囊泡为ATCC 33277。这些结果表明，牙龈假单胞菌rgp A基因的HGP44、HGP15、HGP17和HGP27外膜复合体与中间假单胞菌共聚集有关，这些复合体可能具有多个中间假单胞菌结合位点。

Coaggregation is one the colonization mechanism of oral bacteria. It is observed that Porphyromonas gingivalis coaggregated with Prevotella intermedia which is recognized not only periodontal pocket but also in normal gingival crevice. Some putative coaggregation factor were purified from the vesicle of P.gingivalis. One of the coaggregation factor gene was cloned from the chromosomal DNA of P.gingivalis ATCC 33277. This putative coaggregation factor gene sequence had homology with HGP 17domain gene of rgp A gene. Fusion protein solution of GST-HGP17protein did not aggregate the P.intermedia ATCC 25611. However, P.intermedia ATCC 25611 adhered to the Glutathione Sepharose 4B bead which bind with GST-HGP 17 protein. P.intermedia ATCC25611 adhere to the Glutathione Sepharose 4B bead which bind with GST-HGP44, GST-HGP15, GST-HGP27, respectively. It had homology of amino acid sequence among HGP44, HGP17, and HGP27 and HGP15. P.intermedia ATCC 25611 adhere to the both Glutathion Sepharose 4B bead which bound GST-HGP44F, internal region of HGP44 which had homology with HGP17 and 27, and GST-HGP44R, internal region of HGP44 which had homology with HGP15. Monoclonal antibody against HGP 44 only weakly inhibited the aggregation of P.intermediate ATCC 25611 with the vesicle of P.gingival is ATCC 33277.These results suggest that outer membrane complex of HGP44, HGP15, HGP17, and HGP27 from rgp A gene of P.gingivalis are associated with the coaggregation with P.intermedia and these complex may have several binding site for P.intermedia.