Natural antisense of GTP cyclohydrolase 1 : physiological functions and expression mechanisms of the antisense RNA

GTP环化水解酶1的天然反义：反义RNA的生理功能和表达机制

• 批准号: 19592157
• 负责人: NAKANISHI Nobuo
• 金额: $ 2.91万
• 依托单位: Meikai University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2007
• 资助国家: 日本
• 起止时间: 2007 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-19592157/
• 关键词: 内在性アンチセンスRNA; GTPシクロヒドロラーゼ; 遺伝子発現調節; テトラヒドロビオプテリン; 一酸化窒素; 神経伝達物質; トランスクリプトーム; natural antisense RNA; GTP cyclohydrolase 1; tetrahydrobiopterin; nitric oxide synthase; RNA interference; transcriptome; microRNA; post-transcriptional regulation

Tetrahydrobiopterin (BH4) is an essential cofactor for the enzymes which synthesize monoamine neurotransmitters and nitric oxide, thus influencing neuronal and vascular functions. We found in PC12 cells and in rat and mouse livers natural antisense RNA (naRNA) of GTP cyclohydrolase1 (GCH), a rate-limiting enzyme in BH4 de novo synthesis. When the PC12 cells were stimulated by various agents which increased the cellular BH4 level, GCH naRNA was increased in parallel with GCH mRNA.

四氢生物蝶呤(BH4)是合成单胺类神经递质和一氧化氮的酶的重要辅因子，从而影响神经和血管功能。我们在PC12细胞以及大鼠和小鼠肝脏中发现了GTP环水解酶1(GCH)的天然反义RNA(NANA)，GCH是BH4从头合成的限速酶。PC12细胞在不同浓度的BH4刺激下，GCH-NANA与GCH-mR-NA呈平行增加。