新規同定されたＮＨ細胞におけるサイトカイン産生を制御する転写ネットワークの解明

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We proposed to characterize the transcriptional landscapes of naive, IL2+IL25 and IL33 activated NH cells. RNA from FACS-sorted cells were sequenced using helicosCAGE. The 2-10 million CAGE tags obtained were mapped and their 5'extremity representing Transcription Start Sites were clustered (TSS clusters) and associated with RefSeq transcripts. In parallel, our collaborators investigated IL33 induced signaling, uncovering the role of GATA3 (Furusawa etal. J Immunol. 2013), which we focused our attention on. We uncovered 36 stimulated NH cell-specific GATA3 targets. TSS clusters location, associations and expression levels are available via the ZENBU browser (Severin etal. Nat Biotech. Accepted) which serves for the dissemination of the FAMTOM5 project, in which this study was incorporated.