新規同定されたＮＨ細胞におけるサイトカイン産生を制御する転写ネットワークの解明

阐明新发现的控制 NH 细胞细胞因子产生的转录网络

• 批准号: 23710238
• 负责人: BERTIN nicolas
• 金额: $ 2.83万
• 依托单位: The Institute of Physical and Chemical Research
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23710238/
• 关键词: gene networks; transcriptome; natural helper cell; sytokine; transcriptional network; CAGE; immune system; 遺伝子ネットワーク; 免疫; トランスクリプトーム

We proposed to characterize the transcriptional landscapes of naive, IL2+IL25 and IL33 activated NH cells. RNA from FACS-sorted cells were sequenced using helicosCAGE. The 2-10 million CAGE tags obtained were mapped and their 5'extremity representing Transcription Start Sites were clustered (TSS clusters) and associated with RefSeq transcripts. In parallel, our collaborators investigated IL33 induced signaling, uncovering the role of GATA3 (Furusawa etal. J Immunol. 2013), which we focused our attention on. We uncovered 36 stimulated NH cell-specific GATA3 targets. TSS clusters location, associations and expression levels are available via the ZENBU browser (Severin etal. Nat Biotech. Accepted) which serves for the dissemination of the FAMTOM5 project, in which this study was incorporated.

我们提出表征幼稚，IL2+IL25和IL33激活的NH细胞的转录景观。使用helicosCAGE对facs分选细胞的RNA进行测序。将获得的2- 1000万个CAGE标签进行映射，其5'末端代表转录起始位点（Transcription Start Sites）被聚类（TSS簇），并与RefSeq转录本相关联。同时，我们的合作者研究了IL33诱导的信号传导，揭示了GATA3的作用（Furusawa等）。J Immunol. 2013)，这是我们关注的重点。我们发现了36个受刺激的NH细胞特异性GATA3靶点。TSS集群的位置、关联和表达水平可以通过ZENBU浏览器（Severin等）获得。Nat生物技术。已接受)，用于传播FAMTOM5项目，本研究已纳入该项目。