耳石欠損マウスによる耳石形成・吸収メカニズムの解明

阐明耳石缺陷小鼠的耳石形成和吸收机制

• 批准号: 24791756
• 负责人: 本田 圭司
• 金额: $ 1.66万
• 依托单位: Tokyo Medical and Dental University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2012
• 资助国家: 日本
• 起止时间: 2012-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-24791756/
• 关键词: 遺伝子; 神経科学; 耳石; Atp2b2

形質膜カルシウムポンプ遺伝子(Atp2b2)変異マウスの１つであるWriggle Mouse Sagami(WMS)は耳石欠損マウスとして知られており、出生直後には耳石を有しており、加齢と共に消失・脱落することが推定されている。本年度の研究として、WMSマウスの耳石消失過程において有毛細胞の変化を共焦点レーザー顕微鏡にて観察した。WMSマウスの迷路骨包を採取し、4%パラホルムアルデヒドで固定後、卵形嚢および球形嚢平衡斑を採取し、Allexa 568 Phalloidinにて蛍光染色し、Carl Zeisss社製LSM510で有毛細胞感覚毛の分布、形態を観察した。日齢21日のホモ接合体において、球形嚢および卵形嚢平衡斑の有毛細胞はともに変性や脱落を認めなかった。一方日齢90日のホモ接合体では、球形嚢平衡斑の有毛細胞は大部分が脱落していたが、卵形嚢平衡斑は正常な形態を保っていた。ヘテロ接合体では、日齢21日から90日の期間で平衡斑は正常であった。前年度の研究成果として、マイクロフォーカスCTや実体顕微鏡での観察にて、ホモ接合体において日齢21日で球形嚢の耳石が消失し、日齢90日で卵形嚢の耳石も消失したことを報告した。この結果と合わせると、Atp2b2変異マウスは一度耳石が生成された後に加齢とともに耳石が消失し、その後遅れて有毛細胞が変性・脱落すること、およびその過程は卵形嚢と球形嚢とで異なることが示唆された。したがって、Atp2b2遺伝子は耳石の維持機構において重要な役割を担っていると考えられた。

The shape of the membrane is not known to the child (Atp2b2). The otolith is not known to the Wriggle Mouse Sagami (WMS). After birth, the otolith is not known to the child, and it is presumed to have disappeared after birth. This year, we have studied the disappearance of otolith in WMS cells in the course of this year's study. After the labyrinthine bone bag was removed, the WMS was fixed, the ovate, the spherical, the balanced spot, the Allexa 568 Phalloidin, the light staining, the Carl Zeisss LSM510, the hairy cell, the hair, the shape, the hair, the hair, the shape, the distribution of the hair, the distribution of the hair, On the 21st, the conjugative body, the oval, the balanced spot, the hairy cell, the sexual exfoliation, the globular, the oval, the hairy, the ovoid, the hairy, the ovoid, the hairy, the hairy, the ovoid, the hairy, the hairy, the shedding, the shedding. On one side, on the 90th day, most of the hairy cells were shedding, and the shape of the oval "balance spot" was normal. The balance of the joint will be normal during the 90th day of the 21st and 21st. In the previous year, the results of the study were reviewed, and the results of the study were reported in the previous year, including the results of the research, the results of the previous year, the results of the research, the results of the previous year, the results of the research, the results of the research, the results of the previous year, the results of the research, the results of the research, the results of the previous year, the results of the research, the results of the research, the results of the previous year, the results of the research, the results of the research, the results of the previous year, the results of the previous year, the results of the research, the results of the research, the results of the previous year, the Results the results showed that the otolith disappeared after the formation of the otolith, and the hairy cells showed signs of sexual exfoliation after the formation of the oval, the Atp2b2, the oval, the globular, the oval, the oval, the ovoid, the oval, the oval, the ovoid, the oval, the It is necessary to maintain the important service of the organization by using the otolith of the Atp2b2 machine.

项目成果

A Morphological Study of Otolith Organs with X-ray microtomograph

X射线显微断层扫描耳石器官的形态学研究

• 发表时间: 2012
• 作者: Keiji Honda;Yoshihiro Noguchi;Yoshiyuki Kawashima;Masatoki Takahashi;Ayako Nishio;Nobutaka Osawa;Ken Kitamura
• 通讯作者: Ken Kitamura

マイクロCTを用いた耳石器の形態解析

使用显微 CT 进行耳石器官的形态学分析

• 发表时间: 2012
• 作者: 本田圭司;野口佳裕;川島慶之;西尾綾子;喜多村健
• 通讯作者: 喜多村健