Optimisation of cell culture and molecular typing for the characterisation of Cryptosporidium in water.

优化细胞培养和分子分型以表征水中隐孢子虫。

• 批准号: LP0347126
• 负责人: Prof Richard Thompson
• 金额: $ 21.79万
• 依托单位: Murdoch University
• 依托单位国家: 澳大利亚
• 项目类别: Linkage Projects
• 财政年份: 2003
• 资助国家: 澳大利亚
• 起止时间: 2003-09-26 至 2008-12-31
• 项目状态: 已结题
• 来源: https://dataportal.arc.gov.au/RGS/Web/Grants/LP0347126
• 关键词: Optimisation; cell; culture; molecular; typing

Cryptosporidium is an important waterborne agent of diarrhoeal disease in people and animals. Improved methods for accurately detecting viable parasite stages recovered from water are a priority research need for the water industry. They are dependent upon improved in vitro cultivation procedures that can be combined with DNA-based assays for identifying species and strains of Cryptosporidium and closely related protozoa recovered from freshwater. This project addresses these needs in a collaborative study between scientists at Murdoch University with expertise in both in vitro cultivation and genetic characterisation of Cryptosporidium, and scientists from two of the major water utilities in Australia.

隐孢子虫是一种重要的人畜共患病的水媒病原体。 改进的方法，准确地检测可行的寄生虫阶段回收的水是一个优先的研究需要的水工业。 它们依赖于改进的体外培养程序，该程序可以与基于DNA的测定相结合，用于鉴定从淡水中回收的隐孢子虫和密切相关的原生动物的物种和菌株。 该项目在默多克大学的科学家之间的合作研究中解决了这些需求，这些科学家在隐孢子虫的体外培养和遗传特性方面具有专业知识，并且来自澳大利亚两个主要水务公司的科学家。