PeptiMatrix: Next generation peptide hydrogels for 3D cell culture

PeptiMatrix：用于 3D 细胞培养的下一代肽水凝胶

• 批准号: 10070302
• 金额: $ 34.49万
• 依托单位: PEPTIMATRIX
• 依托单位国家: 英国
• 项目类别: Collaborative R&D
• 财政年份: 2023
• 资助国家: 英国
• 起止时间: 2023 至 无数据
• 项目状态: 未结题
• 来源: https://gtr.ukri.org/projects?ref=10070302
• 关键词: PeptiMatrix; Next; generation; peptide; hydrogels

Current _in vitro_ models of development and disease typically fail to recapitulate the complexities of native tissue microenvironments, leading to a poor extrapolation of _in vitro_ findings to _in vivo_ relevance. This is a major contributing factor to the high attrition rate of new drugs moving from the lab to the clinic, leading to an estimated cost of US$2 billion to bring a new lifesaving therapy to market.Growing recognition of this has led to a recent interest in developing more sophisticated 3D _in vitro_ model platforms which allow for customisation of matrix stiffness and composition. However, despite considerable research effort, no single platform has yet pulled ahead of the competition. With the support of the BBSRC and NC3Rs, we have developed a fully synthetic self-assembling peptide hydrogel (SAPH) platform which aims to address all the current shortcomings of available 3D _in vitro_ model platforms.PeptiMatrix(tm) is chemically well-defined, has limited batch variability, and contains no irrelevant animal-derived material. It can also be customised via a variety of available methods to match the stiffness and composition of any target tissue type. Each formulation of PeptiMatrix(tm) will, therefore, have been specifically tailored to model a specific tissue or tissue type and validated in a relevant cell-based assay.PeptiMatrix(tm) is supplied ready-to-use: simply combine with your cells of interest and plate out. Your cells will then cluster and grow in 3D, in a physiologically relevant and reproducible way. This saves time versus similar products that require complex preparatory steps or require that you customise (and optimise) the hydrogel yourself.

目前的体外发育和疾病模型通常无法概括天然组织微环境的复杂性，导致体外发现与体内相关性的外推较差。这是导致新药从实验室转移到临床的高损耗率的主要因素，导致将新的救命疗法推向市场的估计成本为20亿美元。人们越来越认识到这一点，最近人们对开发更复杂的3D _体外_模型平台产生了兴趣，该平台允许定制基质刚度和成分。然而，尽管进行了大量的研究工作，但还没有一个平台在竞争中领先。在BBSRC和NC3R的支持下，我们开发了一种全合成的自组装肽水凝胶（SAPH）平台，旨在解决现有3D _in vitro_ model平台的所有缺点。PeptiMatrix（tm）化学性质明确，批次可变性有限，不含无关的动物源性材料。它还可以通过各种可用的方法进行定制，以匹配任何目标组织类型的硬度和成分。因此，PeptiMatrix（tm）的每种配方都是专门定制的，以模拟特定的组织或组织类型，并在相关的基于细胞的测定中进行验证。PeptiMatrix（tm）是随时可用的：用途：只需与您感兴趣的细胞联合收割机结合并铺板。然后，您的细胞将以生理相关和可重复的方式在3D中聚集和生长。与需要复杂准备步骤或需要您自己定制（和优化）水凝胶的类似产品相比，这节省了时间。