PeptiMatrix: Next generation peptide hydrogels for 3D cell culture

目前的体外发育和疾病模型通常不能概括自然组织微环境的复杂性，导致体外研究结果与体内相关性的推断很差。这是导致新药从实验室转移到临床的高磨损率的主要因素，导致将一种新的救命疗法推向市场的估计成本为20亿美元。对此的日益认识导致了最近对开发更复杂的3D体外模型平台的兴趣，该平台允许定制基质硬度和成分。然而，尽管做出了相当大的研究努力，但还没有一家平台在竞争中领先。在BBSRC和NC3Rs的支持下，我们开发了一种全合成自组装多肽水凝胶(SAPH)平台，旨在解决现有3D体外模型平台的所有缺点。PeptiMatrix(Tm)化学定义良好，批次可变性有限，不含无关的动物衍生材料。它还可以通过各种可用的方法进行定制，以匹配任何目标组织类型的硬度和组成。因此，PeptiMatrix(Tm)的每一种配方都将专门为特定的组织或组织类型建模，并在相关的基于细胞的分析中得到验证。PeptiMatrix(Tm)提供现成的产品：只需与您感兴趣的细胞结合，即可取出。然后，你的细胞将以一种生理上相关和可复制的方式在3D中聚集和生长。与需要复杂的准备步骤或需要您自己定制(和优化)水凝胶的类似产品相比，这节省了时间。

Current _in vitro_ models of development and disease typically fail to recapitulate the complexities of native tissue microenvironments, leading to a poor extrapolation of _in vitro_ findings to _in vivo_ relevance. This is a major contributing factor to the high attrition rate of new drugs moving from the lab to the clinic, leading to an estimated cost of US$2 billion to bring a new lifesaving therapy to market.Growing recognition of this has led to a recent interest in developing more sophisticated 3D _in vitro_ model platforms which allow for customisation of matrix stiffness and composition. However, despite considerable research effort, no single platform has yet pulled ahead of the competition. With the support of the BBSRC and NC3Rs, we have developed a fully synthetic self-assembling peptide hydrogel (SAPH) platform which aims to address all the current shortcomings of available 3D _in vitro_ model platforms.PeptiMatrix(tm) is chemically well-defined, has limited batch variability, and contains no irrelevant animal-derived material. It can also be customised via a variety of available methods to match the stiffness and composition of any target tissue type. Each formulation of PeptiMatrix(tm) will, therefore, have been specifically tailored to model a specific tissue or tissue type and validated in a relevant cell-based assay.PeptiMatrix(tm) is supplied ready-to-use: simply combine with your cells of interest and plate out. Your cells will then cluster and grow in 3D, in a physiologically relevant and reproducible way. This saves time versus similar products that require complex preparatory steps or require that you customise (and optimise) the hydrogel yourself.