Construction of antibody-based molecular imaging techniques to analyze a development of tumor angiogenesis.

我们的目的是建立一种利用抗肿瘤内皮标记抗体的抗体成像方法来分析肿瘤血管的进展。在这些抗体的评估中，我们发现细胞内化抗体可以有效地积累到内皮细胞中。这一特性可能有助于肿瘤内皮细胞的特异性标记。在一种新型磁共振显像剂的开发中，我们发现聚乙二醇修饰的金氧化铁纳米颗粒可以在肿瘤中积聚，并且它们可以通过MRI显示肿瘤组织。此外，我们还开发了一种在PEG尖端修饰抗体的方法。它可能是一种新的肿瘤内皮特异性MR显像剂。我们还开发了一种使用123I标记抗体的方法。在大阪大学Hatazawa博士的帮助下，我们正在挑战基于抗体的SPECT成像。在未来，这些基于抗体的技术将有助于分析肿瘤血管的进展。

We aim to develop a method to analyze a progress of tumor blood vessels by antibody-based imaging using anti-tumor endothelial marker antibodies. In the assessment of these antibodies, we show that cell-internalizing antibody can accumulate into the endothelial cells efficiently. This property may be useful for specific labeling of tumor endothelial cells. In the development of a novel MR imaging agent, we show that PEG-modified gold-iron oxide nanoparticles can accumulate in the tumor, and they can visualize a tumor tissue using MRI. In addition, we also developed a method to modify antibodies on a tip of PEG. It may be novel tumor endothelial specific MR imaging agent. We also developed a method of antibody labeling using 123I. We are challenging an antibody based SPECT imaging with help of Dr. Hatazawa, Osaka University. In the future, these antibody-based technologies will be useful for the analysis of the progress of tumor blood vessels.