Development immunomodulative drug based on the recognition mechanism of viral B-DNA.

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Previous our data have discovered the novel H2B function to recognize non-chromosomal double stranded DNA (dsDNA), and induce antiviral innate immune response. In current research, we constructed the fusion protein, named as N'-CARD, by fusion of IPS-1 fragment with H2B fragment, and found that N'-CARD induced type I interferons (Type I IFNs) production. In addition, nuclear DNA helicase II was identified as N'-CARD associated molecules, and was indispensable for N'-CARD-mediated innate immune response. Furthermore, N'-CARD fused to protein transduction domain (N'-CARD-PTD) is a candidate of novel adjuvant. Therefore, current research revealed H2B-sensing machinery of cytoplasmic dsDNA as well as novel adjuvant candidate for vaccine development.