Biomolecular analysis on disturbance of glomerular filtration at the early stage of diabetes

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We have visualised the early augmented glomerular filtration in-vivo that may be related to the development of diabetic nephropathy with albuminuria by multi-photon microscopy. We hypothesised that there are structural and component protein distributional changes, which may be associated with albuminuria.We used control rats and diabetic Wistar rats. After kidneys were extracted, slit membrane component proteins. Stained images of each component were binarised and the distributions of slit membrane proteins were analysed by area ratio. Area ratio was found decreasing linearly with diabetic duration. Amongst pair combinations of 3 proteins, the best correlation was between the diabetic duration and the area ratio of nephrin and podocin (r=0.871). This means discrepancy of slit membrane protein distribution deteriorates progressively.