Biomolecular analysis on disturbance of glomerular filtration at the early stage of diabetes

糖尿病早期肾小球滤过紊乱的生物分子分析

• 批准号: 23650278
• 负责人: NAKAMOTO Hiroshi
• 金额: $ 2.33万
• 依托单位: Kawasaki Medical School
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23650278/
• 关键词: 糖尿病; 糸球体濾過; C-peptide, ポドサイト; スリット膜蛋白; ネフリン; ポ ドシン; CD2AP; C-peptide; ポドサイト; ポドシン; スリットダイアフラム; 濾過

We have visualised the early augmented glomerular filtration in-vivo that may be related to the development of diabetic nephropathy with albuminuria by multi-photon microscopy. We hypothesised that there are structural and component protein distributional changes, which may be associated with albuminuria.We used control rats and diabetic Wistar rats. After kidneys were extracted, slit membrane component proteins. Stained images of each component were binarised and the distributions of slit membrane proteins were analysed by area ratio. Area ratio was found decreasing linearly with diabetic duration. Amongst pair combinations of 3 proteins, the best correlation was between the diabetic duration and the area ratio of nephrin and podocin (r=0.871). This means discrepancy of slit membrane protein distribution deteriorates progressively.

我们已经通过多光子显微镜观察到体内早期肾小球滤过增强，这可能与蛋白尿的糖尿病肾病的发展有关。我们假设有结构和组成蛋白分布的变化，这可能与蛋白尿有关。我们使用对照大鼠和糖尿病Wistar大鼠。肾脏提取后，切开细胞膜成分蛋白。对每一组分的染色图像进行二值化，并用面积比分析裂隙膜蛋白的分布。面积比随病程的延长呈线性下降。在3种蛋白质的配对组合中，糖尿病病程与neparin/podocin面积比的相关性最好(r=0.871)。这意味着裂隙膜蛋白分布的差异逐渐恶化。

项目成果

Wide inter-footprocess area in a rat at the early stage of diabetes

糖尿病早期大鼠足突间面积宽

• 发表时间: 2013
• 作者: Nobuhide Doi;Koichi Kakukawa;Yo Oishi;and Hiroshi Yanagawa;Hiroshi Nakamoto
• 通讯作者: Hiroshi Nakamoto

Quantitative analysis on structural change of slit membrane proteins

狭缝膜蛋白结构变化的定量分析

• 发表时间: 2012
• 作者: Yasuda S;Yanagi T;Yamada MD;Ueki S;Maruta S;Inoue A;Arata T.;Hiroshi Nakamoto
• 通讯作者: Hiroshi Nakamoto

Progressive dissociation of nephrin and podocin, glomerular slit membrane proteins, in distribution at the early stage of diabetes

肾小球裂隙膜蛋白去氧肾上腺素和足多蛋白的渐进解离，在糖尿病早期的分布

• 发表时间: 2012
• 作者: Kumiko Ishikawa;Yuhki Tamura;Shinsaku Maruta;Hiroshi Nakamoto
• 通讯作者: Hiroshi Nakamoto

In Vivo Quantitative Visualization Analysis of the Effect of C-Peptide on Glomerular Hyperfiltration in Diabetic Rats by Using Multiphoton Microscopy

• DOI: 10.1111/micc.12043
• 发表时间: 2013-07-01
• 期刊: MICROCIRCULATION
• 影响因子: 2.4
• 作者: Nakamoto, Hiroshi;Kajiya, Fumihiko
• 通讯作者: Kajiya, Fumihiko

Distributional Changes of Glomerular Slit Membrane Components and Widening of Inter-foot Process Spaces in Early Diabetic Rats

早期糖尿病大鼠肾小球缝膜成分的分布变化和足间突间隙的扩大

• 发表时间: 2012
• 作者: Ishikawa;K.;Tohyama;K.;Mitsuhashi;S.;& Maruta;S.;Kenichi Horisawa and Hiroshi Yanagawa;Hiroshi Nakamoto
• 通讯作者: Hiroshi Nakamoto