Research on novel degradation enzymes and genes essential to microbial degradation of persistent chlorinated organophosphorus triesters

微生物降解持久性氯化有机磷三酯的新型降解酶和基因研究

• 批准号: 20310039
• 负责人: KERA Yoshio
• 金额: $ 11.73万
• 依托单位: Nagaoka University of Technology
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20310039/
• 关键词: 環境保全技術; 難分解性有害物; 含塩素有機リン酸トリエステル類; 新規分解微生物; 新規分解酵素(ホスホトリエステラーゼ); 遺伝子クローニング; 遺伝子発現; 遺伝子破遺伝子破壊株; 微生物分解; 新規トリエステラーゼ; 遺伝子破壊株; 新規分解酵素(トリエステラーゼ); 新規分解酵素; トリエステラーゼ; 遺伝子; クローニング; 単離・精製; 特徴解析

The chlorinated organophosphorus(OP) compounds tris(2-chloroethyl) phosphate(TCEP) and tris(1, 3-dichloro-2-propyl) phosphate(TDCPP) are widely used as flame retardants mainly in a range of plastic foams, resins, and latexes and in the production of liquid unsaturated polyester resins, respectively. They are hard to eliminate not only in the environment but also in sewage treatment plants. Many studies have shown several toxic effects of the compoundsIn this study, novel bacteria degrading trihaloalkyl phosphates, Sphingomonas sp. strain TDK1 and Sphingobium sp. strain TCM1, were isolated from soil by growth on TDCPP or TCEP as a sole phosphorus source, respectively. The two strains were able to degrade and assimilate not only the chlorinated OP flame retardants but also several other OP flame retardants and plasticizers, and the strains possessed different substrate specificity for trialkyl phosphates. We purified and characterized phosphotriesterases, catalyzing the first step of TCEP and TDCPP degradation, from Sphingobium sp. TCM1 and Sphingomonas sp. TDK1, respectively. The corresponding genes of the enzymes were cloned and analyzed. The results suggested that both the enzymes were very closely related each other and very distantly related to phosphotriesterases so far reported.

氯化有机磷化合物三(2-氯乙基)磷酸(TCEP)和三(1，3-二氯-2-丙基)磷酸(TDCPP)分别被广泛用作阻燃剂，主要用于各种塑料泡沫、树脂和乳胶以及液体不饱和聚酯树脂的生产。它们不仅在环境中很难消除，在污水处理厂也是如此。在本研究中，新发现的降解三卤烷基磷酸盐的细菌--神经鞘氨醇单胞菌(Sphingomonas sp.)菌株TDK1和Sphingobium sp.菌株TCM1分别以TDCPP和TCEP为唯一磷源从土壤中分离得到。这两株菌株不仅能降解和同化氯化OP阻燃剂，还能降解和吸收其他几种OP阻燃剂和增塑剂，而且对三烷基磷酸酯具有不同的底物专一性。我们从Sphingobium sp.分离纯化并鉴定了催化TCEP和TDCPP降解第一步的磷酸三酯酶。TCM1和鞘氨醇单胞菌。TDK1。对这些酶的相应基因进行克隆和分析。结果表明，这两种酶的亲缘关系非常密切，而与已报道的磷酸三酯酶的亲缘关系很远。

项目成果

Tris(2-chloroethyl)phosphate-degrading characteristics of Sphingobium sp. TCM1 and purification of the degrading enzyme.

鞘氨醇三(2-氯乙基)磷酸酯的降解特性。

• 发表时间: 2008
• 作者: Satoshi Yoshida;Yuka Doi;Jun-ichi Mori;Yuto Suzuki;Tomoki Obana;Katsumasa Abe;Shouji Takahashi;Yoshio Kera
• 通讯作者: Yoshio Kera

Characterization of tris(2-chloroethyl) phosphate-degrading bacterium Sphingobium sp. TCM1 and purification of the degrading Enzyme

三(2-氯乙基)磷酸酯降解菌鞘氨醇菌的表征。

• 发表时间: 2009
• 作者: Satoshi Yoshida;Yuka Doi;Jun-ichi Mori;Yuto Suzuki;Yuki Obana;Katsumasa Abe;Shouji Takahashi;and Yoshio Kera
• 通讯作者: and Yoshio Kera

Sphingobium sp.TCM1株ハロアルキルリン酸加水分解酵素遺伝子の機能解析

Sphingobium sp.TCM1菌株卤代烷基磷酸水解酶基因的功能分析

• 发表时间: 2011
• 作者: Md. Mofazzal Hossain;Yaochun Yao;Takayuki Watanabe;間島亮介・阿部勝正・高橋祥司・解良芳夫
• 通讯作者: 間島亮介・阿部勝正・高橋祥司・解良芳夫

Sphingobium sp. TCM1ハロアルキルリン酸加水分解酵素遺伝子破壊株の構築

Sphingobium sp. TCM1 卤烷基磷酸水解酶基因破坏菌株的构建

• 发表时间: 2011
• 作者: 上原伸夫;吉田蔵;清水得夫;阿部勝正・間島亮介・高橋祥司・解良芳夫
• 通讯作者: 阿部勝正・間島亮介・高橋祥司・解良芳夫

Sphingomonas sp. TDK1 株 tris(1,3-dichloro-2-propyl)phosphate 分解酵素遺伝子のクローニングと大腸菌における発現

鞘氨醇单胞菌TDK1菌株三(1,3-二氯-2-丙基)磷酸降解酶基因的克隆及在大肠杆菌中的表达

• 发表时间: 2010
• 作者: 齋藤誠;齋藤徹;平出正孝;山田裕里恵・川上和延・鈴木雄斗・阿部勝正・高橋祥司・解良芳夫
• 通讯作者: 山田裕里恵・川上和延・鈴木雄斗・阿部勝正・高橋祥司・解良芳夫