Structural analysis of genomic regions where genetic recombination was suppressed for over million years

对基因重组被抑制数百万年的基因组区域进行结构分析

• 批准号: 20310118
• 负责人: ABE Kuniya
• 金额: $ 12.4万
• 依托单位: The Institute of Physical and Chemical Research
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20310118/
• 关键词: ゲノム進化; 再編; 染色体組換え; t-ハプロタイプ; BACライブラリー; ゲノム再編成; ゲノム進化・再編

Goal of this study is to elucidate the structure and divergence of t-haplotype genome in which genetic recombination has been suppressed for over million years. Large scale DNA rearrangements including deletions and duplications were revealed by Array-CGH method, inferring the break points of inversions associated with t-haplotypes. BAC genomic library was constructed from tw5 haplotype, and clones corresponding to the t-complex region were isolated and sequenced. Exceptionally high incidence of SNPs (single nucleotide polymorphisms) has been detected between the tw5haplotype and the standard laboratory mouse strain.

本研究的目的是阐明t-单倍型基因组的结构和分歧，其中遗传重组已被抑制超过百万年。利用Array-CGH方法揭示了大规模的DNA重排，包括缺失和重复，推断了与t单倍型相关的倒位断点。从tw 5单倍型构建BAC基因组文库，分离对应于t-复合物区域的克隆并测序。异常高的SNPs（单核苷酸多态性）的发病率已被检测到tw 5单倍型和标准实验室小鼠品系。

项目成果

New evidence confirms that the mitochondrial bottleneck is generated without reduction of mitochondrial DNA content in early primordial germ cells of mice.

• DOI: 10.1371/journal.pgen.1000756
• 发表时间: 2009-12
• 期刊: PLoS genetics
• 影响因子: 4.5
• 作者: Cao L;Shitara H;Sugimoto M;Hayashi J;Abe K;Yonekawa H
• 通讯作者: Yonekawa H

Highly parallel SNP genotyping reveals high-resolution landscape of mono-allelic Ube3a expression associated with locus-wide antisense transcription.

• DOI: 10.1093/nar/gkq1201
• 发表时间: 2011-04
• 期刊: Nucleic acids research
• 影响因子: 14.9
• 作者: Numata K;Kohama C;Abe K;Kiyosawa H
• 通讯作者: Kiyosawa H

Differences and similarities in the developmental status of embryo-derived stem cells and primordial germ cells revealed by global expression profiling

全局表达谱揭示胚胎干细胞和原始生殖细胞发育状态的异同

• 发表时间: 2008
• 期刊: Genes to cells 13-8
• 作者: Mise;N.;et. al.
• 通讯作者: et. al.

Large-scale production of growing oocytes from neonatal mouse ovaries

从新生小鼠卵巢中大规模生产生长中的卵母细胞

• 期刊: Int. J. Dev. Biol. (in press)
• 作者: Honda;A.;et. al.
• 通讯作者: et. al.

Evaluations of functional processes in live animals by in vivo imaging.

通过体内成像评估活体动物的功能过程。

• 发表时间: 2009
• 作者: Liqin CAO;Kuniya ABE
• 通讯作者: Kuniya ABE