Mechanism of abnormal protein aggregation and the study of the repair enzyme.

已观察到来自年龄相关疾病如白内障、年龄相关性黄斑变性和日光性弹性组织变性的蛋白质的异常聚集。在这项研究中，我们研究了生物学上不常见的D-氨基酸在白内障的眼睛透镜和紫外线损伤的皮肤的老年人。紫外线照射和氧化胁迫加速了蛋白质中D-氨基酸的形成。我们提出D-氨基酸可能是蛋白质异常聚集的触发因素之一，并可诱导相应蛋白质的部分解折叠，导致疾病状态。我们提出了一种新的方法，用于分析异构化的个别天冬氨酸残基的蛋白质，使用LC-MS。这使得有可能分析异构体的天冬氨酸残基在蛋白质中准确和快速。

Abnormal aggregation in protein from age-related diseases such as cataracts, age-related macular degeneration and solar elastosis has been observed. In this study, we examined the biologically uncommon D-amino acids in the cataractous eye lens and UV-damaged skin of elderly individuals. The formation of the D- amino acids in the protein was accelerated by UV-irradiation and oxidative stress. We proposed that D-amino acids may be one of the triggers for abnormal aggregation of proteins and can induce the partial unfolding of the corresponding proteins, leading to a diseased state. We demonstrated a new method for the analysis of isomerization of individual Asp residues in proteins using LC-MS. This makes it possible to analyze isomers of Asp residues in proteins precisely and quickly.