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Development of the high performance electrofusion device for loading lyo-, cryoprotective agents in vivo

Development of the high performance electrofusion device for loading lyo-, cryoprotective agents in vivo
开发用于体内装载冻干、冷冻保护剂的高性能电融合装置
批准号:
20360094
负责人:
SHIRAKASHI Ryo
金额:
$12.48万
依托单位:
依托单位国家:
日本
项目类别:
Grant-in-Aid for Scientific Research (B)
财政年份:
2008
资助国家:
日本
项目状态:
已结题
起止时间:
2008 至 2010

项目摘要

项目成果

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中文摘要
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英文摘要
The electrofusion of a mammalian cell and a giant unilamellar vesicle encapsulating lyo-, cryoprotectants has been investigated. In this study, a prototype of electrofusion device allowing the fusion one-on-one pair of cell and GUV has been proposed. In addition, the designing method of electrofusion process by calculating the electric field and membrane potential during electrofusion has been developed.
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会议论文
巨大単膜リボソームとJurkat細胞の電気融合に及ぼす電場の影響(パルス電場印加による巨大単膜リポソーム-Jurkat細胞対の電場・膜電位解析と融合率の測定)
电场对巨型单层核糖体和 Jurkat 细胞电融合的影响(电场/膜电位分析以及通过施加脉冲电场测量巨型单层脂质体-Jurkat 细胞对的融合率)
DOI: --
发表时间: 2011
期刊: 日本機械学会論文集
影响因子: --
作者: [白樫了, Randolph Reuss, Alexander Schulz, Vladimir L.Sukhorukov, Ulrich Zimmermann]
通讯作者: Ulrich Zimmermann
トレハロースと細胞凍結・乾燥保存
海藻糖和细胞冷冻/干燥储存
DOI: --
发表时间: 2009
期刊:
影响因子: --
作者: [Hasan, M., Monde, M. and Mitsutake, Y., 白樫了]
通讯作者: 白樫了
Evaluation of FEM Electric Field Analysis Using the Electric Properties of Giant Unilamellar Vesicles (GUV) and Jurkat Cells Measured by Electrorotation Spectroscopy
使用电旋转光谱测量的巨型单层囊泡 (GUV) 和 Jurkat 细胞的电特性评估 FEM 电场分析
DOI: --
发表时间: 2009
期刊: Introduction of Cryo and Lyoprotectants by Electrofusion of GUVs and Living Cells,Heat Transfer Asian Research Vol.38(3)
影响因子: --
作者: [Ryo Shirakashi, Randolph Reuss, Alexander Schulz, Vladimir L. Sukhorukov, Ulrich Zimmermann]
通讯作者: Ulrich Zimmermann
細胞リボソームの誘電分光測定と電場解析
细胞核糖体的介电谱和电场分析
DOI: --
发表时间: 2008
期刊: 第45回日本伝熱シンポジウム講演論文集 2+3
影响因子: --
作者: [白樫了,Randolph Reuss, Alexander Schulz, Vladimir L. Sukhorukov. Ulrich Zimmermann]
通讯作者: Vladimir L. Sukhorukov. Ulrich Zimmermann
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