Densign and Development of Stress Responsive LIPOzyme Reactor Based on Re-functionalization of Junk Peptides

基于垃圾肽重功能化的应激响应性LIPOzyme反应器的设计与开发

• 批准号: 20360350
• 负责人: UMAKOSHI Hiroshi
• 金额: $ 11.98万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20360350/
• 关键词: メンブレン・ストレスバイオテクノロジー; LIPOzyme; 生体膜; バイオリアクター; 人工酵素; ペプチド; リフォールディング; ストレス応答; LIPOzvme

It is demonstrated that the function of LIPOzyme (Liposome+Enzyme) could be induced by the construction of catalytic sites on the liposome membrane as a model biomembrane. The LIPOzyme function could be classified by three categories : (i) electron transfer ; (ii) proton transfer ; (iii) nucleophilic/electrophilic reaction. The LIPOzyme function could be, herewith, controlled by the tuning of junk peptides, ligands or their metal complexes to achieve the desired function. Furthermore, the immobilization of LIPOzyme into the module was presented to develop the novel membrane module. This technique is a promising method to develop the reactor/separator based on the LIPOzyme function. Besides, the mapping of the physicochemical properties of junk peptides and the lipid composition could be performed to clarify the optimal condition for the desired functions of LIPOzyme, resulting in developing their database.

实验结果表明，以脂质体膜为模型生物膜，通过构建催化位点，可以诱导脂质体酶（Liposome+Enzyme）的功能。LIPOzyme的功能可以分为三类：（i）电子转移;（ii）质子转移;（iii）亲核/亲电反应。因此，可以通过调节垃圾肽、配体或其金属络合物来控制LIPOzyme功能以实现所需功能。此外，还提出了将脂质体酶固定化到膜组件中，以开发新型膜组件。该技术是开发基于LIPOzyme功能的反应器/分离器的有前途的方法。此外，可以进行垃圾肽的理化性质和脂质组成的映射，以澄清LIPOzyme的期望功能的最佳条件，从而开发其数据库。

项目成果

LIPOzyme Design Based on Molecular Recognition Function of Liposome

基于脂质体分子识别功能的LIPOzyme设计

• 发表时间: 2008
• 作者: L. Q. Tuan;H. Umakoshi;T. Shimanouchi;R. Kuboi
• 通讯作者: R. Kuboi

Liposome Modified with Mn-Porphyrin Complex can Simultaneously Induce Antioxidative Enzymes-like Activity of Both SuperoxideDismutase and Peroxidase

锰卟啉复合物修饰的脂质体可同时诱导超氧化物歧化酶和过氧化物酶的抗氧化酶样活性

• 发表时间: 2008
• 期刊: Langmuir 24
• 作者: H. Umakoshi;K. Morimoto;Y. Ohama;H. Nagami;T. Shimanouchi;R. Kuboi
• 通讯作者: R. Kuboi

Arsenic (V)Induces a Fluidization of Algal Cell and Liposome Membranes

砷 (V) 诱导藻类细胞和脂质体膜流化

• 发表时间: 2008
• 期刊: T= 22
• 作者: Tuan;L. Q.;T. T. T. Huong;p. T. A. Hong;T. Kawakami;T. Shimanouchi;H. Umakoshi;R. Kuboi
• 通讯作者: R. Kuboi

Preparation of hollow fiber immobilized liposome membrane

中空纤维固定化脂质体膜的制备

• 发表时间: 2009
• 期刊: Membrane 34(5)
• 作者: H.Sugaya;H.Umakoshi;Y.Tohtake;E.Oyama;T.Shimanouchi;R.Kuboi
• 通讯作者: R.Kuboi

Characterization of Oxidized and Fragmented Superoxid DismutaseRecruited on Liposome Surface

脂质体表面招募的氧化和断裂的超氧化物歧化酶的表征

• 发表时间: 2008
• 期刊: Maku (Membrane) 33(4)
• 作者: L.Q. Tuan;H. Umakoshi;T. Shimanouchi;R.Kuboi
• 通讯作者: R.Kuboi