Proton Pumping ATPase and Roles of Acidic pH
Proton Pumping ATPase and Roles of Acidic pH
批准号:
20370048
负责人:
FUTAI Masamitsu
金额:
$12.98万
依托单位:
依托单位国家:
日本
项目类别:
Grant-in-Aid for Scientific Research (B)
财政年份:
2008
资助国家:
日本
项目状态:
已结题
起止时间:
2008 至 2010
中文摘要
我们研究了生理条件下f -ATP合成酶和v -ATP酶的旋转机制。得到了重要的生化和热力学调控机制。我们还研究了v - atp酶在破骨细胞囊泡运输和质膜形成中的作用。研究了多核细胞的形成和破骨细胞的分化。
英文摘要
We have studied rotary mechanism of F-ATPase (ATP synthase) and V-ATPase (lysosome-type ATPase) under the physiological conditions. Significant results were obtained for biochemical and thermodynamic regulatory mechanism. We also studied roles of V-ATPase in vesicle trafficking and formation of osteoclast plasma membrane. Formation of multinuclear cells was studied together with osteoclast differentiation.
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Temperature dependence of single molecule rotation of the Escherichia coli ATP synthase F_1 sector reveals the importance of α・β subunit interactions in the catalytic dwell.
大肠杆菌 ATP 合酶 F_1 部分的单分子旋转的温度依赖性揭示了 α·β 亚基相互作用在催化停留中的重要性。
DOI:
--
发表时间:
2009
期刊:
J.Biol.Chem. 284
影响因子:
--
作者:
[M. Sekiya, R.K. Nakamoto, M.K. Al-Shawi, M. Nakanishi-Matsui, M. Futai]
通讯作者:
M. Futai
The effects of γ subunit Met23 replacement on ATP synthase F_1 sector rotation.
γ 亚基 Met23 替换对 ATP 合酶 F_1 扇区旋转的影响。
DOI:
--
发表时间:
2008
期刊:
影响因子:
--
作者:
[Kim, J., Motomiya, Y., Ueda, M, Nakamura, M., Misumi, Y., Saito, S., Ikemizu, S., Misumi, S., Ota, K., Shinriki, S., Kai, H., Ando, Y., 関谷瑞樹]
通讯作者:
関谷瑞樹
ATP合成酵素のβ-γサブユニット間相互作用による回転への影響(口頭)
ATP 合酶 β-γ 亚基之间的相互作用对旋转的影响(口服)
DOI:
--
发表时间:
2008
期刊:
影响因子:
--
作者:
[Kamiyama, S., 関谷瑞樹]
通讯作者:
関谷瑞樹
DOI:
10.1074/jbc.m109.009019
发表时间:
2009-06
期刊:
The Journal of Biological Chemistry
影响因子:
--
作者:
[Mizuki Sekiya;R. Nakamoto;M. Al-Shawi;M. Nakanishi‐Matsui;M. Futai]
通讯作者:
Mizuki Sekiya;R. Nakamoto;M. Al-Shawi;M. Nakanishi‐Matsui;M. Futai
Thermodynamics of single molecule rotation of Escherichia coli F-ATPase
大肠杆菌F-ATP酶单分子旋转的热力学
DOI:
--
发表时间:
2010
期刊:
影响因子:
--
作者:
[Sekiya M., Hosokawa H., Nakanishi-Matsui M., Al-Shawi MK., Nakamoto RK., Futai M.]
通讯作者:
Futai M.
共 53 条
Vacuolar System Organelles and Inhibitors
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批准号:08307018
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项目类别:Grant-in-Aid for Scientific Research (A)
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资助金额:$13.18万
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财政年份:1996
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负责人:FUTAI Masamitsu
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依托单位:
Mechanism of H^+ transport by H^+ATPase
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批准号:07458159
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项目类别:Grant-in-Aid for Scientific Research (B)
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资助金额:$4.67万
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财政年份:1995
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负责人:FUTAI Masamitsu
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依托单位:
Mechanism of Energy Coupling between Catalysis (ATP Synthesis and Hydrolysis) and H^+ Transport in ATP Synthase
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批准号:05454630
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项目类别:Grant-in-Aid for General Scientific Research (B)
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资助金额:$4.1万
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财政年份:1993
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负责人:FUTAI Masamitsu
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依托单位:
Studies on H^+-ATPase:Catlytic site and Coupling between Catalysis
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批准号:03454546
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项目类别:Grant-in-Aid for General Scientific Research (B)
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资助金额:$4.1万
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财政年份:1991
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负责人:FUTAI Masamitsu
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依托单位:
Mechanism of Ion Transport by H^+-ATPase and H^+/K^+-ATPase
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批准号:01480528
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项目类别:Grant-in-Aid for General Scientific Research (B)
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资助金额:$3.9万
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财政年份:1989
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负责人:FUTAI Masamitsu
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依托单位:
Structure and Catalytic Mechanism of Ion-translocating ATPases
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批准号:62480458
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项目类别:Grant-in-Aid for General Scientific Research (B)
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资助金额:$3.84万
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财政年份:1987
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负责人:FUTAI Masamitsu
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依托单位:
Mechanism of ATP Synthase (F F ): Analysis at the level of amino acid residues of subunits.
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批准号:60480501
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项目类别:Grant-in-Aid for General Scientific Research (B)
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资助金额:$0.77万
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财政年份:1985
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负责人:FUTAI Masamitsu
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依托单位:
海外基金