Vacuolar System Organelles and Inhibitors

植物或动物细胞具有内核体、溶酶体、突触囊泡和高尔基体等膜细胞器。它们参与胞吞或胞吐的过程。不同背景和学科的科学家进行了这个项目，交流了技术和想法。取得的主要成果有：(1)在松果体细胞中发现了新的细胞器（微泡），并对其性质进行了广泛的研究。(2)在细菌中发现了新的转运Na^+的囊泡型atp酶。(3)我们发现了质子通路亚基（蛋白脂）的同工型。(4)详细研究了脂质在细胞器间的转运。(5)研究了不同来源的液泡型atp酶抑制剂。这些结果有助于了解液泡型atp酶在不同细胞中的作用，并有助于发现可能具有药用价值的化学物质。

Plant or animal cells have endomembrane organelles such as endosome, lysosome, synaptic vesicles and Golgi apparatus. They participate in the process of endocytosis or exocytosis. Scientists with different back ground and discipline carried out this project exchanging techniques and ideas. Major results obtained are : (1) new organelle (microvesicles) were identified in pinealocytes and its properties were extensively studied. (2) New vesicle type ATPase transporting Na^+ was found in bacteria. (3) We found isoforms of proton pathway subunits (proteolipid). (4) Transport of lipids between organelles was studied in detail. (5) We studied inhibitors for vacuolar tyoe ATPase from different origins. These results contribute greatly to the understanding on the role of vacuolar type ATPase in different cells and will contribute to find possible pharmaceutically valuable chemicals.