Evolutional and functional studies on diversification of virulence genes of plant bacteria with establishment of host species

植物细菌毒力基因多样化随宿主物种建立的进化和功能研究

• 批准号: 20380029
• 负责人: HIKICHI Yasufumi
• 金额: $ 10.32万
• 依托单位: Kochi University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20380029/
• 关键词: 病理学; 進化; 農林水産物; 遺伝学

Pseudomonas cichorii induces bacterial rot and necrotic spot in lettuce and eggplant. P. cichorii required the hrp genes (hrp) for its full virulence on eggplant. An aldehyde dehydrogenase gene (aldH) and a phosphinothricin N-acetyltransferase gene (pat) are located in the flanking region of hrp and were involved in the bacterial virulence on eggplant, independently of hrp. The phylogenetic analysis showed that P. cichorii strains acquires hrp and pat through horizontal transfer from a common ancestor with the S-PAI of P. viridiflava and implicate hrp and pat with aldH in its virulence. Inoculation into Asteraceae species showed that involvement of hrp, aldH and pat in P. cichorii virulence had no relationship with the phylogeny of the Asteraceae species. Therefore, P. cichorii might implicate not only hrp but also aldH and pat in its virulence on respective plant species.

菊苣假单胞菌可引起生菜和茄子的细菌性腐烂和坏死斑。 P. cichorii 需要 hrp 基因 (hrp) 才能对茄子产生完全毒力。乙醛脱氢酶基因 (aldH) 和草胺膦 N-乙酰转移酶基因 (pat) 位于 hrp 的侧翼区域，独立于 hrp 参与茄子的细菌毒力。系统发育分析表明，菊苣 P. cichorii 菌株通过与 P. viridiflava 的 S-PAI 的共同祖先水平转移获得了 hrp 和 pat，并表明 hrp 和 pat 与 aldH 的毒力有关。菊科物种的接种表明，hrp、aldH和pat参与菊苣毒力与菊科物种的系统发育无关。因此，菊苣 P. cichorii 可能不仅与 hrp 相关，还与 aldH 和 pat 对各自植物物种的毒力有关。

项目成果

Pseudomonas cichoriiのナスヘの病原性に関するaldehyde dehydrogenase遺伝子の機能的および系統進化学的解析

菊苣假单胞菌对茄子致病性的乙醛脱氢酶基因功能及系统发育分析

• 发表时间: 2008
• 作者: 田中将之;小柳諒;大西浩平;中屋敷均;木場章範;曵地康史
• 通讯作者: 曵地康史

The hrp genes of Pseudomonas cichorii are essential for pathogenicity on eggplant but not on lettuce.

• DOI: 10.1099/mic.0.2008/021097-0
• 发表时间: 2008-10
• 期刊: Microbiology (Reading, England)
• 作者: Hojo H;Koyanagi M;Tanaka M;Kajihara S;Ohnishi K;Kiba A;Hikichi Y
• 通讯作者: Hikichi Y

Regulation mechanism of hrp regulon with a new positive regulator PrhG in Ralstonia solanacearum

青枯菌中新型正调节因子PrhG对hrp调节子的调控机制

• 发表时间: 2009
• 作者: Hayakawa T.;Shimizu Y.;Ishida T.;Sakai H.;Kohei Kambara;田中将之;Yong Zhang
• 通讯作者: Yong Zhang

The amino acid substitution at position 740 in σ70 of Ralstonia solanacearum strain OE1-1 affects its in planta growth

青枯雷尔斯顿菌OE1-1 σ70位点740位氨基酸取代影响其在植物中的生长

• 发表时间: 2008
• 期刊: Appl. Environ. Microbiol. 74
• 作者: Kanda;A.;Tsuneishi;K.;Mori;A.;Ohnishi;K.;Kiba;A.;Hikichi;Y.
• 通讯作者: Y.

Pseudomonas viridiflava, a causal agent of bacterial rot of lettuce, requires both a pectate lyase gene and hrp genes for its virulence

绿色假单胞菌 (Pseudomonas viridiflava) 是生菜细菌性腐烂病的致病菌，需要果胶酸裂解酶基因和 HRP 基因才能发挥毒力

• 发表时间: 2010
• 作者: Hiruma;K.;and Takano;Y.;高尾和実;塩見邦博;Yui Hikida
• 通讯作者: Yui Hikida