The hrp genes of Pseudomonas cichorii are essential for pathogenicity on eggplant but not on lettuce.
The hrp genes of Pseudomonas cichorii are essential for pathogenicity on eggplant but not on lettuce.
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DOI：
10.1099/mic.0.2008/021097-0
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发表时间：
2008-10
期刊：
影响因子：
--
通讯作者：
Hikichi Y
中科院分区：
文献类型：
--
作者：
Hojo H;Koyanagi M;Tanaka M;Kajihara S;Ohnishi K;Kiba A;Hikichi Y
菊苣假单胞菌引起茄子的坏死病变和莴苣的腐烂。通过将转座子插入到P. cichorii菌株SPC 9018中，我们产生了两个突变体，4-57和2-99，它们对茄子而不是生菜失去了毒性。分析表明，转座子插入到4-57中的hrpG基因和2-99中的hrcT基因中。其hrp基因的核苷酸序列与绿黄假单胞菌BS群菌株的hrp基因的核苷酸序列同源。4-57对茄子的致病性通过用hrpF操纵子转化而恢复，所述hrpF操纵子源自SPC 9018或BS组成员绿黄单胞菌菌株9504（Pv 9504）。这些数据表明hrp基因参与了SPC 9018对茄子的致病性，并且hrpF操纵子在SPC 9018和Pv 9504之间具有功能保守性。hrpS突变体和hrpL突变体都不能引起茄子叶片的坏死性病变，但保留其对莴苣的致病性。这些结果表明，番茄疫霉在茄子上的致病性依赖于hrp，而在莴苣上的致病性不依赖于hrp。
Pseudomonas cichorii causes necrotic lesions in eggplant and rot in lettuce. Through transposon insertion into P. cichorii strain SPC9018 we produced two mutants, 4-57 and 2-99, that lost virulence on eggplant but not lettuce. Analyses showed that a transposon was inserted into the hrpG gene in 4-57 and the hrcT gene in 2-99. Nucleotide sequences of the hrp genes of SPC9018 are homologous to those of Pseudomonas viridiflava BS group strains. The pathogenicity of 4-57 on eggplant was restored by transformation with an hrpF operon, originating from either SPC9018 or the BS group member P. viridiflava strain 9504 (Pv9504). These data suggested the involvement of hrp genes in the pathogenicity of SPC9018 on eggplant, and functional conservation of hrpF operons between SPC9018 and Pv9504. Both the hrpS mutant and the hrpL mutant were unable to cause necrotic lesions on eggplant leaves but retained their pathogenicity against lettuce. These results suggest that the pathogenicity of P. cichorii is hrp-dependent in eggplant, but not in lettuce.
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影响因子：
3.2
作者：
STASKAWICZ, B;DAHLBECK, D;NAPOLI, C
通讯作者：
NAPOLI, C
DOI：
10.1073/pnas.1731982100
发表时间：
2003-09-02
影响因子：
11.1
作者：
Buell, CR;Joardar, V;Collmer, A
通讯作者：
Collmer, A
DOI：
10.3186/jjphytopath.62.125
发表时间：
1996-01-01
期刊：
Annals of the Phytopathological Society of Japan
影响因子：
--
作者：
Hikichi, Yasufumi;Saito, Akira;Suzuki, Kazumi
通讯作者：
Suzuki, Kazumi
影响因子：
4.3
作者：
Kiba, Akinori;Takata, Osamu;Hikichi, Yasufumi
通讯作者：
Hikichi, Yasufumi
影响因子：
2.8
作者：
Yamamoto, S;Kasai, H;Harayama, S
通讯作者：
Harayama, S