Development of a synthetic method for hydrophobic glycoprotein and its application to saposin synthesis

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The synthesis of highly hydrophobic glycoproteins, such as membrane proteins and lipid-binding proteins, is extremely difficult even with the use of efficient segment coupling methods. The main difficulty is that the segments usually retain low solubility during peptide synthesis, purification and ligation steps. To overcome this problem, a method to enhance the solubility of segments during peptide synthesis and purification was developed and applied to the synthesis of lipid-binding protein, saposin C.