A breakthrough to the microheterogeneity of the carbohydrate structure in glycoprotein by synthetic approach

合成方法突破糖蛋白碳水化合物结构微观异质性

基本信息

  • 批准号:
    16580093
  • 负责人:
  • 金额:
    $ 1.92万
  • 依托单位:
  • 依托单位国家:
    日本
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
  • 财政年份:
    2004
  • 资助国家:
    日本
  • 起止时间:
    2004 至 2005
  • 项目状态:
    已结题

项目摘要

Carbohydrates on proteins play various essential roles, such as cell-growth, differentiation, and tumor metastasis. However, the details of these functions are not understood at a molecular level, which mainly arises from the microheterogeneity at the carbohydrate portions. Especially, mucins retain a dense cluster of highly heterogeneous carbohydrates, which makes its functional analysis difficult.In this research, we developed a method to prepare glycopeptides carrying different carbohydrates at distinct amino acid residues to mimic the microheterogeneity of the natural glycoproteins, which will provide a basis to solve the problem arising from the microheterogeniety. First, we synthesized a glycopeptide derived from the consensus sequence of MUC5AC. During the solid-phase synthesis, Fmoc-Ser and Fmoc-Thr carrying core 8 carbohydrate were introduced at specific positions, giving the MUC5AC glycopeptide carrying two carbohydrates at different amino acid residues. In the second example … More , a novel approach to the glycopeptide carrying a couple of distinctively sialylated core 2 O-glycan was developed. The sequence derived from the leukosialin was divided into two shorter peptide segments. The N-terminal peptide thioester carrying one core 2 O-glycan was synthesized using the modified procedure for the Fmoc peptide thioester preparation. C-terminal peptide amide carrying a core 2 O-glycan was prepared by the conventional Fmoc method. The N- and C-terminal peptide was treated with different sialyl transferases giving two differently sialylated glycopeptides. These two segments were then condensed by the thioester method to give a glycopeptide carrying a couple of distinctively sialylated core 2 glycan. In the third example, a method to synthesize larger glycopeptide models of mucins was developed. The sequence derived from the repeating unit of MUC2, carrying 7 O-GalNAc moieties at specific positions, was prepared by the modified procedure for the Fmoc peptide thioester preparation. This peptide was then repeatedly condensed 6 times by the thioester method to obtain glycoprotein composed of 141 amino acid residues carrying 42 GalNAc moieties. At present, this is the largest glycoprotein prepared by the fully chemical procedure. The method can be used to synthesize mucin models carrying different carbohydrates at distinct positions.The synthetic heterogeneously glycosylated peptides prepared by the above procedures will be useful to understand the microheterogeniety of glycoproteins, especially of mucins. Less
蛋白质上的碳水化合物起着各种重要作用,如细胞生长、分化和肿瘤转移。然而,这些功能的细节在分子水平上还不清楚,这主要是由于碳水化合物部分的微观异质性引起的。特别是粘蛋白中存在大量的高度异质性的糖链,这给粘蛋白的功能分析带来了困难,本研究通过制备不同氨基酸残基上携带不同糖链的糖肽来模拟天然糖蛋白的微观异质性,为解决这种微观异质性所带来的问题提供了基础。首先,我们从MUC 5AC的共有序列合成了一个糖肽。在固相合成过程中,在特定位置引入携带核心8糖的Fmoc-Ser和Fmoc-Thr,得到在不同氨基酸残基处携带两个糖的MUC 5AC糖肽。在第二示例中 ...更多信息 本研究开发了一种新的制备带有两个唾液酸化的核心2 O-聚糖的糖肽的方法。衍生自白唾液酸的序列被分成两个较短的肽段。使用Fmoc肽硫酯制备的改进程序合成携带一个核心2 O-聚糖的N-末端肽硫酯。通过常规Fmoc方法制备携带核心2 O-聚糖的C-末端肽酰胺。用不同的唾液酸转移酶处理N-和C-末端肽,得到两种不同的唾液酸化糖肽。然后通过硫酯法将这两个片段缩合,得到携带一对独特唾液酸化的核心2聚糖的糖肽。在第三个实施例中,开发了合成粘蛋白的较大糖肽模型的方法。通过用于Fmoc肽硫酯制备的改进程序制备衍生自MUC 2的重复单元的序列,其在特定位置携带7个O-GalNAc部分。然后通过硫酯法将该肽重复缩合6次,以获得由141个氨基酸残基组成的糖蛋白,其携带42个GalNAc部分。这是目前全化学法制备的最大的糖蛋白。该方法可用于合成在不同位置携带不同糖基的粘蛋白模型,所合成的糖基化多肽将有助于理解糖蛋白,特别是粘蛋白的微观异质性。少

项目成果

期刊论文数量(28)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Synthesis of a mimic for the heterogeneous surface of core 2 sialoglycan-linked glycoprotein
  • DOI:
    10.1021/ol048827b
  • 发表时间:
    2004-09-02
  • 期刊:
  • 影响因子:
    5.2
  • 作者:
    Takano, Y;Hojo, H;Nakahara, Y
  • 通讯作者:
    Nakahara, Y
Solid-phase synthesis of core 8 O-glycan-linked MUC5AC glycopeptide.
核心 8 O-聚糖连接的 MUC5AC 糖肽的固相合成。
  • DOI:
  • 发表时间:
    2005
  • 期刊:
  • 影响因子:
    0
  • 作者:
    M.Maehara;A.Ohgaki;Y.Nakahara;H.Hojo;Y.Nakahara.
  • 通讯作者:
    Y.Nakahara.
Application of Fmoc-amino acid carrying an unmasked carbohydrate to th e synthesis of the epidermal growth factor-like domain o f bovine blood coaguladon factor IX,
携带未掩蔽碳水化合物的Fmoc-氨基酸在合成牛血凝固因子IX的表皮生长因子样结构域中的应用,
  • DOI:
  • 发表时间:
    2004
  • 期刊:
  • 影响因子:
    0
  • 作者:
    T.Takemura;H.Hojo et al.
  • 通讯作者:
    H.Hojo et al.
Chemical Synthesis of 23 kDa Glycoprotein by Repetitive Segment Condensation : A Synthesis of MUC2 Basal Motif Carrying Multiple O-GalNAcMoieties.
通过重复片段缩合化学合成 23 kDa 糖蛋白:携带多个 O-GalNAc 部分的 MUC2 基础基序的合成。
  • DOI:
  • 发表时间:
    2005
  • 期刊:
  • 影响因子:
    0
  • 作者:
    H.Hojo;Y.Matsumoto;Y.Nakahara;E.Ito;Y.SUzuki;M.Suzuki;A.Suzuki;Y.Nakahara.
  • 通讯作者:
    Y.Nakahara.
Synthesis of a glycosylated peptide thioester by the Boc strategy and its application to segment condensation
  • DOI:
    10.1271/bbb.50621
  • 发表时间:
    2006-06-01
  • 期刊:
  • 影响因子:
    1.6
  • 作者:
    Haginoya, Eiichiro;Hojo, Hironobu;Watanabe, Yasushi
  • 通讯作者:
    Watanabe, Yasushi
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HOJO Hironobu其他文献

HOJO Hironobu的其他文献

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{{ truncateString('HOJO Hironobu', 18)}}的其他基金

Synthesis of complex-type glycan containing LacdiNAc structure and its application to the glycoprotein synthesis
含LacdiNAc结构的复合型聚糖的合成及其在糖蛋白合成中的应用
  • 批准号:
    23380065
  • 财政年份:
    2011
  • 资助金额:
    $ 1.92万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Development of a synthetic method for hydrophobic glycoprotein and its application to saposin synthesis
疏水性糖蛋白合成方法的建立及其在皂苷合成中的应用
  • 批准号:
    20380069
  • 财政年份:
    2008
  • 资助金额:
    $ 1.92万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
In vitro folding of synthetic glycoprotein
合成糖蛋白的体外折叠
  • 批准号:
    18580107
  • 财政年份:
    2006
  • 资助金额:
    $ 1.92万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Synthesis of novel cyclodextrin derivatives and its application to search for the inhibitor of H.pyroli growth
新型环糊精衍生物的合成及其在寻找H.pyroli生长抑制剂中的应用
  • 批准号:
    14560085
  • 财政年份:
    2002
  • 资助金额:
    $ 1.92万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)

相似海外基金

Synthesis of oligosaccharides by using solid-phase method and hybrid method of solid-phase and liquid-phase
固相法及固相与液相混合法合成低聚糖
  • 批准号:
    15310149
  • 财政年份:
    2003
  • 资助金额:
    $ 1.92万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Photochemical Reactions and the Solid Phase Method
光化学反应和固相法
  • 批准号:
    7610893
  • 财政年份:
    1977
  • 资助金额:
    $ 1.92万
  • 项目类别:
    Standard Grant
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