Proteomics of bile acid signals

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According to proteomics approach, we tried to develop an analytical method for bile acid signal transduction and clarify the function of bile acids in the brain. The results by the affinity labeling analysis of growth hormone using chenodeoxycholyl adenylate showed that chenodeoxycholate binds to the region different from the growth hormone receptor binding site. Next, we developed an analytical method of phosphorylated proteins using the specific derivatization and the double pseudo-neutral loss extraction on nanoLC/ESI-MS/MS. We found its usefulness for identification of phosphorylated proteins in complex protein mixtures and identification of phosphorylated sites. In addition, we extracted chenodeoxycholate-binding proteins in hepatocytes by using the chenodeoxycholate-immobilized cleavable affinity gel, and identified dihydrodiol dehydrogenase, which is known as a bile acid-binding protein in the hepatocyte, and peroxiredoxin 1.