Development of a noninvasive transdermal gene delivery system for gene therapy of diabetes

在这项研究中，我们试图通过人工基因传递系统MEND的无创透皮给药，在皮肤和肌肉中产生胰岛素，从而开发糖尿病的基因治疗技术。我们成功地通过喷射注射实现了MEND的无创经皮递送，并在皮肤中诱导了持续的基因表达。我们构建了胰岛素前原质粒DNA，并证实mRNA在培养细胞和皮肤中有显著表达。此外，经MEND治疗的糖尿病模型小鼠胰岛素分泌增加。

In this study, we attempted to develop gene therapeutic technology for diabetes by production of insulin in skin and muscle via noninvasive transdermal delivery of artificial gene delivery system MEND. We succeeded the noninvasive transdermal delivery of MEND by jet-injection, and induction of sustained gene expression in the skin. We constructed preproinsulin plasmid DNA, and confirmed significant expression of the mRNA in the culture cells and skin. Moreover, increment of insulin production in diabetes-model mice treated with MEND.