Development of a noninvasive transdermal gene delivery system for gene therapy of diabetes

在这项研究中，我们试图开发糖尿病的基因治疗技术，通过人工基因递送系统MEND的无创经皮给药，在皮肤和肌肉中产生胰岛素。我们成功地通过喷射注射无创地经皮给药，并在皮肤中诱导了持续的基因表达。构建了胰岛素前体质粒DNA，证实该基因在培养细胞和皮肤中均有显著表达。此外，用MEND治疗糖尿病模型小鼠的胰岛素产生增加。

In this study, we attempted to develop gene therapeutic technology for diabetes by production of insulin in skin and muscle via noninvasive transdermal delivery of artificial gene delivery system MEND. We succeeded the noninvasive transdermal delivery of MEND by jet-injection, and induction of sustained gene expression in the skin. We constructed preproinsulin plasmid DNA, and confirmed significant expression of the mRNA in the culture cells and skin. Moreover, increment of insulin production in diabetes-model mice treated with MEND.