Investigation of clinical impacts of CNV in the pharmacokinetic-related genes

CNV 对药代动力学相关基因的临床影响研究

• 批准号: 20390048
• 负责人: IEIRI Ichiro
• 金额: $ 12.4万
• 依托单位: Kyushu University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20390048/
• 关键词: 薬物動態; 遺伝子; コピー数多型; CNV; 薬剤反応性; PK; PD遺伝子; 肺癌

We developed a new method for the analysis of CNV, and analyzed of CNV in PK/PD-related genes in healthy volunteers and NSCLC patients. The new method, using real-time PCR and calibrator vector, was high-throughput to determine the absolute copy number, and it was expected to suit for the clinical diagnosis. But, it is necessary to take account of the localization of target sequence of primer and probes. We detected some new CNV in PK/PD related genes. As the result of the analysis in detail, some may change the function of the gene. The clinical impacts of CNV should be cleared by further investigations.

我们建立了一种新的CNV分析方法，并对健康志愿者和NSCLC患者的PK/PD相关基因进行了CNV分析。该方法采用实时荧光定量PCR和校准品载体，可高通量测定绝对拷贝数，有望应用于临床诊断。但是，必须考虑引物和探针的靶序列的定位。我们在PK/PD相关基因中检测到一些新的CNV。作为详细分析的结果，有些可能会改变基因的功能。CNV的临床影响应通过进一步研究来明确。

项目成果

Real-time PCR法を用いたpharmacokinetics/pharmacodynamics関連遺伝子のCopy Number Variation (CNV)解析修士論文

使用实时PCR方法进行药代动力学/药效相关基因的拷贝数变异（CNV）分析硕士论文

• 发表时间: 2009
• 作者: Fujii-Kuriyama;Y.Kawajiri;K.;et al.;木下智広
• 通讯作者: 木下智広

Systematic Screening of Human ABCC3 Polymorphisms and Their Effects on MRP3 Expression and Function

• DOI: 10.2133/dmpk.dmpk-10-rg-103
• 发表时间: 2011-01-01
• 期刊: DRUG METABOLISM AND PHARMACOKINETICS
• 影响因子: 2.1
• 作者: Sasaki, Tomohiro;Hirota, Takeshi;Ieiri, Ichiro
• 通讯作者: Ieiri, Ichiro