Cohesin-dependentregulation of the expression of drug transporter genes

人肠道CYP 3A 4和ABCB 1 mRNA表达水平显示出很大的个体间差异和它们之间的显着关联。采用亚硫酸氢盐测序法对CYP 3A 4和ABCB 1基因位点之间的CpG岛进行分析。在人肠道样品中，一些CG位点（编号14-18）部分脱甲基。由于这些区域包括CTCF结合区，因此CG位点（No.14 -18）的去甲基化被认为是CYP 3A 4和ABCB 1基因座内聚介导转录绝缘的重要因素。

Human intestinal CYP3A4 and ABCB1 mRNA expression levels showed large inter-individual differences and a significant association between them. The CpG island located in the region between CYP3A4and ABCB1 genes locus were analyzed by bisulfite sequencing method. Some CG sites (No. 14-18) were partially demethylated in human intestinal samples. Since these regions include CTCF binding regions, demethylation in the CG sites (No. 14-18) are suggested to be important for cohesion mediating transcriptional insulationin CYP3A4 and ABCB1 gene locus.