A new strategy for antibody-therapy against infectious diseases and bacterial terrorism using lymphocyte-chip

利用淋巴细胞芯片针对传染病和细菌恐怖主义进行抗体治疗的新策略

• 批准号: 20390286
• 负责人: MURAGUCHI Atsushi
• 金额: $ 12.06万
• 依托单位: University of Toyama
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20390286/
• 关键词: リンパ球チップ; 抗体医療; 抗体遺伝子; 抗体産生細胞; リコンビナント抗体; インフルエンザウイルス; B型肝炎ウイルス; 中和抗体; 抗体医薬; 感染症; 抗体工学; ヒト抗体; 新興感染症

We developed ISAAC (Immunospot assay for antibody-secreting cell) method that can detect antibody-secreting cells in a microwell using murine system. We then succeeded in generating high-quality human monoclonal antibodies for hepatitis B virus and influenza virus from vaccinated healthy volunteers using the ISAAC method in a very short time ; within one week. We further developed an in vitro assay system to examine the HBV-neutralizing activity of human anti-HBs antibodies and clarified the relationship between neutralizing activity of human mAbs and epitopes of HBs antigen.

我们开发了ISAAC（Immunospot assay for antibody-secreting cell）方法，该方法可以使用小鼠系统在微孔中检测抗体分泌细胞。然后，我们使用ISAAC方法在很短的时间内（一周内）成功地从接种疫苗的健康志愿者中产生了针对B型肝炎病毒和流感病毒的高质量人单克隆抗体。我们进一步建立了人源抗-HBs单克隆抗体的体外中和活性检测体系，并阐明了人源抗-HBs单克隆抗体的中和活性与HBs抗原表位的关系。

项目成果

Enhancement of TRAIL-induced apoptosis with novel TRAIL-R1-specific human mAbs generated by immunospot array assay on a chip.

通过芯片上的免疫点阵列测定产生的新型 TRAIL-R1 特异性人单克隆抗体增强 TRAIL 诱导的细胞凋亡。

• 发表时间: 2010
• 作者: Kishi H;Jin A;Ozawa T;Tajiri K;Lin Z;Obata T;Ishida I;Muraguchi A.
• 通讯作者: Muraguchi A.

Detection of antigen-specific T-cell response on the single cell-basis with microwell array chip.

使用微孔阵列芯片在单细胞基础上检测抗原特异性 T 细胞反应。

• 发表时间: 2010
• 作者: Kishi H;Tajiri K;Hatakeyama S;Kobayashi E;Horii M;Jin A;Ozawa T;Muraguchi A.
• 通讯作者: Muraguchi A.

Rapid and efficient cloning and functional evaluation system for antigen-specific T cell receptor obtained from single T-cells.

从单个 T 细胞获得的抗原特异性 T 细胞受体的快速高效克隆和功能评估系统。

• 发表时间: 2010
• 作者: Horii M;Kobayashi E;Hatakeyama S;Ozawa T;Tajiri K;Kishi H;Muraguchi A.
• 通讯作者: Muraguchi A.

A rapid and efficient single-cell manipulation method for screening antigen-specific antibody-secreting cells from human peripheral blood

• DOI: 10.1038/nm.1966
• 发表时间: 2009-09-01
• 期刊: NATURE MEDICINE
• 影响因子: 82.9
• 作者: Jin, Aishun;Ozawa, Tatsuhiko;Muraguchi, Atsushi
• 通讯作者: Muraguchi, Atsushi

Microwell arrays for detecting single, antigen-specific B-lineage cells

用于检测单个抗原特异性 B 谱系细胞的微孔阵列

• 发表时间: 2008
• 作者: 小澤龍彦;他;Kishi H.
• 通讯作者: Kishi H.