Establishment of personalized immunotherapy method for virus-infected diseases using lymphocyte chip

近年来，禽流感、艾滋病等新发和再发传染病以及其他机会性或潜伏性传染病正威胁着人类。通过病毒特异性单克隆抗体和细胞毒性T细胞的免疫治疗是治疗这些可怕的感染性疾病的最有可能的候选者。我们开发了一种新颖的方法，称为“淋巴细胞芯片”，从感染性疾病患者的外周血中分离人抗原特异性抗体分泌细胞。在本研究中，我们改进了淋巴细胞芯片方法，以有效和快速（10天内）从病毒感染患者以及癌症患者的单个淋巴细胞中克隆抗原特异性抗体或TCR基因，这可能有助于感染性疾病以及癌症的个性化免疫治疗。

Recently, the emerging and re-emerging infectious diseases such as avian flu and AIDS, and other opportunistic or latent infectious diseases are threatening human beings. Immunotherapy via virus-specific monoclonal antibody and cytotoxic T cells is the most likely candidate to treat these fearful infectious diseases. We had developed a novel and innovative method, called "lymphocyte chip", to isolate human antigen-specific antibody-secreting cells from peripheral blood of patients with infectious diseases. In this research, we improved the lymphocyte chip method to clone efficiently and rapidly (within 10 days) antigen specific-antibody or TCR genes from individual lymphocytes of virus-infected patients as well as cancer patients, which may facilitate personalized immunotherapy for infectious diseases as well as cancer.