Effects of early postnatal stress on the development of 5-HT/GABA co-localized neuron in the raphe nuclei

我们通过电生理学、分子生物学和神经解剖学的方法，比较了同样含有GAD67的5-羟色胺能神经元和只含有5-羟色胺的神经元。我们通过原位杂交和单细胞RT-PCR方法证实了色氨酸羟化酶2(TPH2)和GAD67在DRN中共表达。我们还证明，GABA不会释放到这些神经元的突触间隙中。在电生理实验中，我们发现TPH2和GAD67mRNA阳性神经元的动作电位幅度、膜电阻和放电频率均显著低于仅表达TPH2mRNA的神经元。出生后早期应激干扰了TPH2和GAD67mRNA阳性神经元的发育。

We characterized 5-HTergic neurons that also contained GAD67 compared to neurons containing only 5-HT through electrophysiological, molecular biological and neuroanatomical methods. We confirmed tryptophan hydroxylase 2 (TPH2) mRNA and GAD67 mRNA co-expression in the DRN via in situ hybridization and single-cell RT-PCR methods. We also demonstrated that GABA is not released into the synaptic cleft of these neurons. In the electrophysiological experiment, we found that the action potential amplitude, membrane resistance and firing frequency of both TPH2 and GAD67 mRNA-positive neurons were all significantly lower than in neurons that were positive for TPH2 mRNA only. An early postnatal stress disturbed the development of TPH2 and GAD67 mRNA-positive neurons.