Functional and morphological study for cross-talk among the monoaminergic nervous system and the developmental mechanism of heteroreceptors.

单胺能神经元向许多靶细胞延伸多分支和发散的连接，几乎所有靶细胞都位于神经元所在的脑区域之外。在这些神经元中，存在着通过异源感受器的相互作用系统。本研究的目的是阐明异源受体的发育机制及其功能。去甲肾上腺素介导的5-羟色胺释放调节存在于大鼠海马。在中缝背核中观察到α 2肾上腺素能受体mRNA。这一结果表明，α 2-肾上腺素受体在肾上腺素能神经末梢发育，并证实α 2-肾上腺素受体调节肾上腺素能神经末梢中的5-羟色胺释放。此外，在中缝背核投射多巴胺能神经末梢的前额叶皮层可能存在通过5-HT 6和5-HT 1B受体调节多巴胺释放的系统。也就是说，氟西汀诱导的多巴胺释放通过用5-HT 6受体mRNA反义核苷酸和5-HT 1B受体拮抗剂处理而减少。

Mono-aminergic neurons extend multiple-branched and divergent connections to many target cells, almost all of which lie outside the brain region in which the neurons are located. Among these neurons there is some cross-talk systems exists via heteroreceptors. The aim of the present study is to elucidate the mechanism of heteroreceptor development and the function.Noradrenaline-mediated regulation in serotonin release exists in the rat hippocampus. Alpha2-adrenoceptor mRNA was observed in the dorsal raphe nucleus. This result indicates that alpha2-adorenoceptors develops at the serotonergic nerve terminals and confirms that alpha2-adorenoceptors regulate serotonin release in the serotonergic nerve terminals. In addition, dopamine release regulation system via 5-HT6 and 5-HT1B receptors might exist in the prefrontal cortex where serotonergic nerve terminals are projected from the dorsal raphe nucleus. Namely, fluoxetine-induced dopamine release is reduced by the treatment with 5-HT6 receptor mRNA antisense nucleotides and a 5-HT1B receptor antagonist.