Functional and morphological study for cross-talk among the monoaminergic nervous system and the developmental mechanism of heteroreceptors.

单胺能神经系统之间的串扰和异质受体发育机制的功能和形态学研究。

• 批准号: 09670085
• 负责人: YOSHIOKA Mitsuhiro
• 金额: $ 1.79万
• 依托单位: HOKKAIDO UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09670085/
• 关键词: serotonin; dopamine; noradrenaline; cross-talk; heteroreceptor; autoreceptor; モノアミン; 系統発生学; シナプス

Mono-aminergic neurons extend multiple-branched and divergent connections to many target cells, almost all of which lie outside the brain region in which the neurons are located. Among these neurons there is some cross-talk systems exists via heteroreceptors. The aim of the present study is to elucidate the mechanism of heteroreceptor development and the function.Noradrenaline-mediated regulation in serotonin release exists in the rat hippocampus. Alpha2-adrenoceptor mRNA was observed in the dorsal raphe nucleus. This result indicates that alpha2-adorenoceptors develops at the serotonergic nerve terminals and confirms that alpha2-adorenoceptors regulate serotonin release in the serotonergic nerve terminals. In addition, dopamine release regulation system via 5-HT6 and 5-HT1B receptors might exist in the prefrontal cortex where serotonergic nerve terminals are projected from the dorsal raphe nucleus. Namely, fluoxetine-induced dopamine release is reduced by the treatment with 5-HT6 receptor mRNA antisense nucleotides and a 5-HT1B receptor antagonist.

单氨基能神经元向许多靶细胞扩展了多支链和不同的连接，几乎所有靶细胞都位于神经元所在的大脑区域之外。在这些神经元中，存在一些跨对词系统，通过异光受体存在。本研究的目的是阐明杂质介导的杂质介导的5-羟色胺释放中的调节，在大鼠海马中存在。在背侧raphe核中观察到α2-肾上腺素受体mRNA。该结果表明α2-粘膜粘膜受体在血清素能神经末端发育，并确认α2-粘膜粘膜应受体调节5-羟色胺能神经末端的5-羟色胺释放。此外，在前额叶皮层中可能存在5-HT6和5-HT1B受体的多巴胺释放调节系统，其中血清素能神经末端从背面raphe核中投射出来。也就是说，用5-HT6受体mRNA反义核苷酸和5-HT1B受体拮抗剂治疗可减少氟西汀诱导的多巴胺释放。

项目成果

Matsumoto, M.: "Effects of idazoxan on dopamine release in the prefrontal cortex of freely moving rats" European Journal of Pharmacology. 343. 165-170 (1998)

Matsumoto, M.：“咪唑克生对自由活动大鼠前额皮质多巴胺释放的影响”欧洲药理学杂志。

Yoshioka, M.: "CENTRAL DISTRIBUTION AND FUNCTION OF 5-HT6 RECEPTOR SUBTYPE IN THE RAT BRAIN" Life Sciences. 62 (17-18). 1473-1477 (1998)

Yoshioka, M.：“大鼠大脑中 5-HT6 受体亚型的中心分布和功能”生命科学。

Yosioka Mitsuhiro: "Effects of idazoxan on dopamine release in the prefrontal cortex of freely moving rats" European Journal of Pharmacology. 343. 165-170 (1998)

Yosioka Mitsuhiro：“达唑克生对自由活动大鼠前额皮质多巴胺释放的影响”欧洲药理学杂志。

Yoshioka, M.: "CENTRAL DISTRIBUTION AND FUNCTION OF 5-HT6 RECEPTOR SUBTYPE IN THE RAT BRAIN" Life Sciences. 62・17-18. 1473-1477 (1998)

Yoshioka, M.：“大鼠大脑中 5-HT6 受体亚型的中心分布和功能”生命科学 62・17-18（1998）。

Yosioka Mitsuhiro: "CENTRAL DISTRIBUTION AND FUNCTION OF 5-HT6 RECEPTOR SUBTYPE IN THE RAT BRAIN" Life Sciences. 62(17-18). 1473-1477 (1998)

Yosioka Mitsuhiro：“5-HT6 受体亚型在大鼠大脑中的中心分布和功能”生命科学。