Investigation of disorders of interaction between bone and bone marrow after intake of alcohol and fat

摄入酒精和脂肪后骨与骨髓相互作用紊乱的研究

• 批准号: 20390406
• 负责人: NAKAMURA Toshitaka
• 金额: $ 11.73万
• 依托单位: University of Occupational and Environmental Health, Japan
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20390406/
• 关键词: アルコール; 脂肪; 骨芽細胞; 骨髄細胞; シグナル伝達; p21; アセトアルデヒド脱水素酵素; アルデヒド脱水素酵素; 皮質骨; 海綿骨; 骨粗鬆症; 骨形成; 骨形態計測; pQCT; wnt; 細胞周期; 卵巣摘出; エストロゲ; p53; オステオカルシン; 海綿骨量; 骨形成率; オステオカルシ; 細胞周期停止

Disruption of Apo E gene with high fat diet resulted in trabecular bone loss and reduced bone formation at the secondary spongiosa of the tibia in mice. In these disrupted mice, apoptosis was induced in osteoblasts through enhanced expression of p53 mRNA and osteoblastic differentiation and proliferation were suppressed. Bone formation was decreased. Disruption of aldehyde-dehydrogenase 2 gene with alcohol intake resulted in trabecular bone loss and reduced bone formation in mice. Cell cycle arrest was induced through enhanced expression of p21 mRNA and osteoblastic mineralization was disturbed. Bone formation was decreased. Thus, we demonstrated the mechanism underlying trabecular bone loss induced by alcohol intake or high fat diet through cell cycle arrest or apoptosis of osteoblasts.

高脂饮食破坏载脂蛋白E基因导致小鼠胫骨松质骨丢失和继发性骨形成减少。在这些破坏的小鼠中，通过增强p53 mRNA的表达诱导成骨细胞凋亡，成骨细胞分化和增殖受到抑制。骨形成减少。在小鼠中，酒精摄入导致的β-脱氢酶2基因的破坏导致骨小梁丢失和骨形成减少。细胞周期阻滞通过p21 mRNA表达的增强而诱导，成骨细胞矿化受到干扰。骨形成减少。因此，我们通过细胞周期阻滞或成骨细胞凋亡证实了酒精摄入或高脂饮食诱导的骨小梁丢失的潜在机制。

项目成果

アルコール負荷後のAldh2ノックアウトマウスでは骨髄細胞がG2期で停止し骨形成が低下する

Aldh2 敲除小鼠酒精负荷后骨髓细胞停滞在 G2 期且骨形成减少

• 发表时间: 2010
• 作者: 清水勇樹;酒井昭典;中村利孝
• 通讯作者: 中村利孝

Skeletal loading enhances osteoblast differentiation and inhibits adipogenic differentiation in bone marrow cells

骨骼负荷增强骨髓细胞中的成骨细胞分化并抑制成脂分化

• 发表时间: 2008
• 作者: Sakai A;Menuki K;Nakamura T
• 通讯作者: Nakamura T

Association of bone mineral density with deformity of the distal radius in low-energy Colies' fractures in Japanese women above 50 years of age.

50 岁以上日本女性低能量 Colies 骨折中骨矿物质密度与桡骨远端畸形的关系。

• 发表时间: 2008
• 期刊: J Hand Surg-AM 33
• 作者: Sakai A;Oshige T;Zenke Y;Suzuki M;Yamanaka Y;Nakamura T;et al.
• 通讯作者: et al.

High-fat diet facilitated decreases in BMD of ovariectomized mice and suppressed the anabolic effects of PTH on bone

高脂饮食促进去势小鼠骨密度下降并抑制PTH对骨的合成代谢作用

• 发表时间: 2008
• 作者: Tanaka S;Sakai A;Nakamura T
• 通讯作者: Nakamura T

High-fat diet facilitated decreases in BMD of ovariectomized mice and suppressed the anabolic effects of PTH on bone.

高脂肪饮食促进去势小鼠骨密度的降低，并抑制 PTH 对骨的合成代谢作用。

• 发表时间: 2008
• 作者: Tanaka S;Sakai A;Nakamura T;et al.
• 通讯作者: et al.