The regulation of cell proliferation and differentiation of bone cells after the change of gravity loading

重力负荷变化对骨细胞增殖和分化的调控

• 批准号: 12470313
• 负责人: NAKAMURA Toshitaka
• 金额: $ 8.77万
• 依托单位: University of Occupational and Environmental Health
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-12470313/
• 关键词: exercise; loading; bone volume; bone strength; bone formation; bone resorption; nitric oxide; CFU-f; 非荷重; 再荷重; 海綿骨; cyclor-oxygenase-2; c-fos; 骨髄細胞; 骨芽細胞; 破骨細胞; 炎症; 2型コラーゲン誘発関節炎; ミネラル沈着結節形成能; 繊維性結節形成能; 慢性荷重; クライミング; iNOS; NO; 尾部懸垂

We comprehensively clarified the sequential changes of bone contour, turnover, and strength after skeletal loading and unloading at the tissue, cellular and molecular levels. The resistant exercise (jumping) increased bone volume and bone strength more effectively by promoting cortical drift and by increasing bone formation, compared to the aerobic exercise (running), in the growing rats.We originally created the voluntarily climbing-up animal model. The trabecular bone volume of the proximal tibia was increased at 2 and 4 weeks after the exercise. Osteoclast surface was decreased at 2 weeks and bone formation was increased at 4 weeks after the exercise. In the cell culture study, the bone marrow cells obtained from the tibias of 2-week exercise mice showed the increase of alkaline phosphatase-positive CFU-f and the decrease of TRAP-positive multinucleated cells. We also revealed that nitric oxide is essential for trabecular bone formation at the reloading after unloading.

我们在组织、细胞和分子水平上全面阐明了骨骼加载和卸载后骨骼轮廓、周转和强度的顺序变化。与有氧运动（跑步）相比，在生长中的大鼠中，抵抗运动（跳跃）通过促进皮质漂移和增加骨形成，更有效地增加了骨体积和骨强度。我们最初创造了自愿攀爬的动物模型。运动后2周和4周，胫骨近端骨小梁体积增加。运动后2周破骨细胞表面减少，4周骨形成增加。在细胞培养研究中，2周运动小鼠胫骨骨髓细胞显示碱性磷酸酶阳性CFU-f增加，trap阳性多核细胞减少。我们还发现，在卸载后再加载时，一氧化氮对小梁骨的形成至关重要。

项目成果

Notomi T, Okimoto N, Nakamura T, et al.: "Tower climbing exercise started 3 months after ovariectomy recovers of bone strenth of the femur and lumbar vertebrae in aged osteopenic rats."Journal of Bone and Mineral Research. 18. 140-149 (2003)

Notomi T、Okimoto N、Nakamura T 等人：“老年骨质疏松大鼠的卵巢切除术后股骨和腰椎骨强度恢复后 3 个月开始爬塔运动。”《骨与矿物质研究杂志》。

Notomi T, Okimoto N, Nakamura T, et al.: "Effects of tower climbing exercise on bone mass, strength, and turnover in growing rats."Journal of Bone and Mineral Research. 16-1. 166-174 (2001)

Notomi T、Okimoto N、Nakamura T 等人：“爬塔运动对生长中大鼠的骨量、力量和骨代谢的影响。”《骨与矿物质研究杂志》。

Ikeda S, Sakai A, Nakamura T et al.: "Effect of trabecular bone contour on ultimate strength of lumbar vertebra after bilateral ovariectomy in rats"Bone. 26・6. 625-633 (2001)

Ikeda S、Sakai A、Nakamura T 等：“骨小梁轮廓对大鼠双侧卵巢切除术后腰椎极限强度的影响”26・6（2001）。

Mori T, Okimoto N, Sakai A, Nakamura T, et al.: "Climbing exercise increases bone mass and trabecular bone turnover through transient regulation of marrow osteogenic and osteoclastogenic potentials in mice."Journal of Bone and Mineral Research. 18-11. 200

Mori T、Okimoto N、Sakai A、Nakamura T 等人：“攀岩运动通过短暂调节小鼠骨髓成骨和破骨细胞潜能来增加骨量和小梁骨转换。”《骨与矿物质研究杂志》。

Masuyama R, Sakai A, Nakamura T, et al.: "Dietary calcium and phosphorus ratio regulates bone mineralization and turnover in vitamin D receptor knockout mice by affecting intestinal calcium and phosphorus absorption."Journal of Bone and Mineral Research.

Masuyama R、Sakai A、Nakamura T 等人：“膳食钙磷比例通过影响肠道钙和磷吸收来调节维生素 D 受体敲除小鼠的骨矿化和周转。”《骨与矿物质研究杂志》。