Analysis of Genomic Aberrations in Osteosarcoma : comparison of age and location

骨肉瘤是最常见的原发性骨实体瘤，其特点是高度的遗传不稳定性，对常规治疗很少有抵抗力。本研究主要探讨染色体畸变和基因表达变化对肿瘤发生部位、组织病理学亚型及预后的影响。我们推测染色体不稳定区的DNA拷贝数改变可能与骨肉瘤的发生有关。应用阵列计算全息技术研究了不同年龄、不同部位骨肉瘤的染色体不稳定性。我们发现四肢患者的DNA缺失率高于躯干患者。我们检测了几个可能导致年轻和老年患者不同生物学致瘤过程的基因区域。

Osteosarcoma is the most common primary solid tumor of bone, which can be characterized by a high level of genetic instability and rarely resistanct to conventional treatments. This study focused on the chromosome aberrations and the changes of gene expressions influencing on the specific tumor site, the histopathological subtypes, and the prognosis. We hypothesized that the DNA copy number changes in the domain of chromosomal instability may be related to the tumorigenesis of osteosarcoma. The chromosomal instabilities of osteosarcoma were studied using the array CGH method for each age and location. We identified that there were more DNA deletion in extremities patients than in trunk patients. We detected several gene regions which may lead to different biological tumorigenic process between young and elderly patients.