Analysis of Genomic Aberrations in Osteosarcoma : comparison of age and location

骨肉瘤是最常见的骨原发实体瘤，具有高度的遗传不稳定性和对常规治疗很少耐药的特点。本研究重点研究染色体异常和基因表达变化对肿瘤的特定部位、组织病理亚型和预后的影响。我们推测染色体不稳定区域的DNA拷贝数变化可能与骨肉瘤的发生有关。用阵列CGH方法研究骨肉瘤各年龄段、不同部位的染色体不稳定性。我们发现四肢患者比躯干患者有更多的DNA缺失。我们检测了几个可能导致青年和老年患者不同生物学肿瘤发生过程的基因区域。

Osteosarcoma is the most common primary solid tumor of bone, which can be characterized by a high level of genetic instability and rarely resistanct to conventional treatments. This study focused on the chromosome aberrations and the changes of gene expressions influencing on the specific tumor site, the histopathological subtypes, and the prognosis. We hypothesized that the DNA copy number changes in the domain of chromosomal instability may be related to the tumorigenesis of osteosarcoma. The chromosomal instabilities of osteosarcoma were studied using the array CGH method for each age and location. We identified that there were more DNA deletion in extremities patients than in trunk patients. We detected several gene regions which may lead to different biological tumorigenic process between young and elderly patients.