Study of molecular mechanisms of post-inflammatory hyperpigmentation and scarless wound healing

炎症后色素沉着与无疤痕愈合的分子机制研究

• 批准号: 20390457
• 负责人: OKAZAKI Mutsumi
• 金额: $ 12.56万
• 依托单位: Tokyo Medical and Dental University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20390457/
• 关键词: 瘢痕形成; 創傷治癒; 部位特異性; 線維芽細胞; 色素沈着; サイトカイン; ケラチノサイト; 遺伝子発現; 表皮角化細胞; 炎症後色素沈着; 解剖学的部位による差異

We investigate anatomical heterogeneity of the fibroblasts using several in vitro assays which are considered to be reflect the fibrotic and melanogenic properties of fibroblasts. Especially, it was shown that fibroblasts obtained from deep dermis showed stronger tendency for wound contraction and extracellular matrix production than those from superficial dermis. The findings suggest that deep dermal fibroblasts were appropriate for compensation of tissue deficit in acute phase wound. Differences between facial and trunk fibroblasts are also investigated using primary fibroblasts obtained from identical donors. As a result, both fibroblasts showed identical proliferative capacities, and it was revealed that trunk fibroblasts showed stronger fibrotic properties than facial fibroblasts. In parallel, trunk fibroblasts showed stronger expression of stem cell factor, one of the representative melanogenic cytokines, than facial fibroblasts. The fact that site-specificities of wound healing experienced in clinical settings are well reflected in cultured fibroblasts suggest that the properties of wound healing is, at least partly, defined with residential fibroblasts.

我们使用几种被认为能反映成纤维细胞的纤维化和黑素生成特性的体外实验来研究成纤维细胞的解剖异质性。尤其是真皮深层成纤维细胞比真皮浅层成纤维细胞具有更强的伤口收缩和细胞外基质生成倾向。结果提示，深部真皮成纤维细胞适合作为急性期创面组织缺损的代偿材料。面部成纤维细胞和躯干成纤维细胞之间的差异也用来自相同捐赠者的原代成纤维细胞进行了研究。结果表明，两种成纤维细胞具有相同的增殖能力，躯干成纤维细胞表现出比面部成纤维细胞更强的纤维化特性。同时，躯干成纤维细胞比面部成纤维细胞表达更强的干细胞因子，干细胞因子是具有代表性的黑素细胞因子之一。事实上，在临床环境中经历的伤口愈合的部位特异性在培养的成纤维细胞中得到了很好的反映，这表明伤口愈合的特性至少部分地由住宅成纤维细胞定义。

项目成果

皮膚、皮下組織および筋組織由来の細胞群の増殖特性

皮肤、皮下组织和肌肉组织细胞群的增殖特性

• 发表时间: 2009
• 作者: 栗田昌和;ほか
• 通讯作者: ほか

Fibroblast obtained from superficial and deep dermis showed different features for wound healing.

从真皮浅层和深层获得的成纤维细胞在伤口愈合方面表现出不同的特征。

• 期刊: J Plast Surg Hand Surg in press
• 作者: Kaminishi-Tanikawa A;Kurita M;Okazaki M;Kawaguchi R;Ihara A;Niikura M;Takushima A;Harii K.
• 通讯作者: Harii K.

顔面および体幹由来線維芽細胞の癒痕形成・色素沈着関連遺伝子発現の違い

面部和躯干成纤维细胞中与疤痕形成和色素沉着相关的基因表达差异

• 发表时间: 2010
• 作者: 栗田昌和、岡崎睦;ほか
• 通讯作者: ほか

耳垂ケロイドおよび健常皮膚の線維芽細胞の増殖特性の比較

耳垂瘢痕疙瘩与健康皮肤成纤维细胞增殖特征比较

• 发表时间: 2010
• 作者: 川口留奈;栗田昌和;岡崎睦;上西昭子;多久嶋亮彦;波利井清紀
• 通讯作者: 波利井清紀

創傷治癒における真皮浅層と深層由来の線維芽細胞の部位特異性

真皮浅层和深层成纤维细胞在伤口愈合中的位点特异性

• 发表时间: 2010
• 作者: 上西昭子;栗田昌和;岡崎睦;川口留奈;多久嶋亮彦;波利井清紀
• 通讯作者: 波利井清紀