Development of novel antiangiogenic therapy based on tumor microenviroenment

为了开发更能反映肿瘤微环境的新型抗血管生成疗法，我们比较了肿瘤内皮细胞（TEC）和正常内皮细胞（NEC）的基因表达，并通过DNA芯片分析鉴定了TEC特异性基因。我们选取了这些基因，并通过siRNA敲除分析了这些分子在tec中的功能。敲除部分基因抑制TEC存活，部分基因抑制TEC上细胞迁移。此外，抑制几种TEC标记物在体内显示肿瘤生长抑制。这些结果表明，它使我们能够开发新的抗血管生成治疗特异性针对TEC。

To develope novel anti-angiogenic therapy reflecting on tumor microenvironment more, we compared gene expression between tumor endothelial cell (TEC) and normal endothelial cell (NEC) and identified TEC specific genes by DNA microarray analysis.We picked up these genes and analyzed the function of these molecules in TECs by knock down using siRNA.Knock down of some genes inhibited TEC survival and some inhibited cell migration on TEC. Furthermore, inhibition of several TEC markers showed tumor growth suppression in vivo. These results suggested that it enabled us to develope novel anti-angiogenic therapy targeting TEC specifically.