Elucidation of onset mechanism of amelogenesis imperfecta (AI) using a biomineralization model

使用生物矿化模型阐明釉质生成不全（AI）的发病机制

• 批准号: 20390522
• 负责人: KOTARO Tanimoto
• 金额: $ 6.16万
• 依托单位: Hiroshima University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20390522/
• 关键词: エナメル蛋白; バイオミネラリゼーション; エナメル質形成不全症; アメロゲニン; ハイドロキシアパタイト

The purpose of this study was to investigate the effects of mutation of amelgenin, an enamel matrix protein, on the enamel crystal formation, and to elucidate of onset mechanism of amelogenesis imperfecta (AI).Wild-type recombinant human full-length amelogenin and mutated recombinant human full-length ameogenin (P41T) were synthesized. There was a difference in the aggregation ability in solution at neutral pH (pH 7.4) between the wild-type and mutated full-length amelogenins. In addition, recombinant human MMP20 wa synthesized, and the digestion of recombinant human full-length amelogenin by the MMP20 was investigated. The digestion of mutated recombinant human full-length amelogenin was delayed as compared with that in wild-type amelogenin.From these results, it was strongly suggested that a mutation of amelogenin may induce the abnormality of enamel structure during tooth formation.

为研究釉质基质蛋白釉原蛋白（amelgenin）突变对釉质晶体形成的影响，阐明釉质形成障碍（amelogenesis alternata，AI）的发生机制，合成了野生型重组人全长釉原蛋白（amelogenin）和突变型重组人全长釉原蛋白（amelogenin，P41 T）。在中性pH（pH 7.4）溶液中，野生型和突变的全长釉原蛋白之间的聚集能力存在差异。此外，还合成了重组人MMP 20，并研究了MMP 20对重组人全长釉原蛋白的酶切作用。与野生型釉原蛋白相比，突变的重组人全长釉原蛋白的消化延迟，这些结果强烈提示釉原蛋白的突变可能导致牙齿形成过程中釉质结构的异常。

项目成果

Amelogenin Modulates the Proliferation of Human Mesenchymal Stem Cells through the MAPK-ERK Pathway

Amelogenin 通过 MAPK-ERK 途径调节人间充质干细胞的增殖

• 发表时间: 2010
• 作者: Y.Miyake;Y.Okamoto;K.Onoda;M Kurosaki;N.Shirao;Y.Okamoto;S.Yamawaki;Huang Yu-ching
• 通讯作者: Huang Yu-ching

Effecs of amelogenin on the proliferation of mesenchymak stem cells

釉原蛋白对间充质干细胞增殖的影响

• 发表时间: 2010
• 作者: 黄郁〓;谷本幸太郎;丹根由起;神谷貴志;犬伏俊博;吉岡基子;道田将彦;笹本智子;吉見友希;岩渕泰憲;丹根一夫
• 通讯作者: 丹根一夫

Effects of human full-length amelogenin on the proliferation of human mesenchymal stem cells derived from bone marrow

• DOI: 10.1007/s00441-010-1064-7
• 发表时间: 2010-11-01
• 期刊: CELL AND TISSUE RESEARCH
• 影响因子: 3.6
• 作者: Huang, Yu-Ching;Tanimoto, Kotaro;Tanne, Kazuo
• 通讯作者: Tanne, Kazuo

Effects of amelogenins on the proliferation and differentiation of human mesenchymal stem cells

牙釉蛋白对人间充质干细胞增殖和分化的影响

• 发表时间: 2009
• 作者: Tomonori Hoshino;Kan Saito;Take Fujiwara;Yu-Ching Huang
• 通讯作者: Yu-Ching Huang

Functional Roles of Prolines at Amelogenin C Terminal during Tooth Enamel Formation

牙釉质形成过程中釉原蛋白 C 末端脯氨酸的功能作用

• 发表时间: 2008
• 期刊: Cells Tissues Organs
• 影响因子: 2.7
• 作者: Li Zhu;K. Tanimoto;T. Le;P. DenBesten;Wu Li
• 通讯作者: Wu Li