Optimization of bone regeneration by use of mesenchymal stem cells and its application for closure of jaw cleft

间充质干细胞优化骨再生及其在颌裂闭合中的应用

• 批准号: 23593029
• 负责人: KOTARO Tanimoto
• 金额: $ 3.24万
• 依托单位: Hiroshima University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23593029/
• 关键词: 歯科矯正学

The purpose of our study is to establish bone regeneration of jaw cleft by use of bone marrow-derived mesenchymal stem cells (MSCs) with a new scaffold, carbonated-hydroxyapatite (CAP). Artificial jaw cleft was created on the both side of maxilla in Beagle dogs. MSCs were derived from bone marrow of iliac bone by use of an aspiration needle, and cultured. After 1 month, MSCs and new scaffold material, CAP granulated powder, were transplanted into the artificial bone defects. Bone regeneration at jaw cleft site was achieved successfully by the transplantation of MSCs/CAP after 3 months. It is also demonstrated suggested that CAP particles could be digested rapidly by osteoclasts. In addition, orthodontic tooth movement into regenerated bone was also achieved. It is emphasized that MSCs/CAP transplantation therapy has a great potential as a new treatment modality for bone regeneration in CLP patients with bone defect due to jaw cleft.

我们的研究目的是利用骨髓间充质干细胞（MSCs）和一种新的支架，碳酸羟基磷灰石（CAP）建立颌骨裂骨再生。在比格犬的上颌骨两侧制造人工颌裂。用抽吸针从髂骨骨髓中提取间充质干细胞，并进行培养。1个月后，将MSCs和新型支架材料CAP颗粒粉移植到人工骨缺损中。MSCs/CAP移植3个月后颌骨裂区骨再生成功。研究还表明，破骨细胞能够快速消化CAP颗粒。此外，还实现了正畸牙齿向再生骨的移动。强调MSCs/CAP移植治疗作为CLP颌骨裂骨缺损患者骨再生的一种新的治疗方式具有很大的潜力。

项目成果

Experimental Tooth Movement Into New Bone Area Regenerated by Use of Bone Marrow-Derived Mesenchymal Stem Cells

• DOI: 10.1597/12-232
• 发表时间: 2015-07-01
• 期刊: CLEFT PALATE-CRANIOFACIAL JOURNAL
• 作者: Tanimoto, Kotaro;Sumi, Keisuke;Tanne, Kazuo
• 通讯作者: Tanne, Kazuo

骨髄由来間葉系幹細胞を用いた顎裂閉鎖治療法の確立-担体の代謝についての検討-

利用骨髓间充质干细胞建立颌裂闭合治疗 - 载体代谢研究 -

• 发表时间: 2012
• 作者: Sumi K.;Tanimoto K.;Tanne Y.;Yoshioka M.;Oki N.;Tanne K;沖奈苗,谷本幸太郎,吉岡基子,鷲見圭輔,吉見友希,杉山勝,丹根由起,丹根一夫;鷲見圭輔,谷本幸太郎,丹根由起,吉岡基子,松田弥生,吉見友希,沖奈苗,杉山勝,丹根一夫
• 通讯作者: 鷲見圭輔,谷本幸太郎,丹根由起,吉岡基子,松田弥生,吉見友希,沖奈苗,杉山勝,丹根一夫

Motoko Yoshioka

吉冈元子

• 发表时间: 2012
• 作者: Bone Regeneration by use of Mesenchymal Stem Cells for Treatment of Jaw Cleft: Part II;Orthodontic Tooth Movement into the Regenerated Bone Area
• 通讯作者: Orthodontic Tooth Movement into the Regenerated Bone Area

Bone regeneration in the treatment of bone defect in the cleft lip and palate patients with mesenchymal stem cells

间充质干细胞治疗唇腭裂患者骨缺损的骨再生

• 发表时间: 2011
• 作者: Tanne K.;Yoshioka M.;Sumi K.;Tanne Y.;Tanimoto K
• 通讯作者: Tanimoto K

Bone regeneration in the treatment of bone defect in the cleft lip and palate patients with mesenchymal stem cells

间充质干细胞治疗唇腭裂患者骨缺损的骨再生

• 发表时间: 2011
• 作者: Tanne K;Yoshioka M;et al.
• 通讯作者: et al.