Optimization of bone regeneration by use of mesenchymal stem cells and its application for closure of jaw cleft

本研究的目的是利用骨髓间充质干细胞（MSCs）与一种新的支架材料碳酸化羟基磷灰石（CAP）复合，建立颌骨裂骨再生模型。在Beagle犬上颌骨两侧建立人工腭裂模型。 通过使用抽吸针从髂骨的骨髓衍生MSC，并进行培养。1个月后，将MSCs和新型支架材料CAP颗粒粉末移植到人工骨缺损中。3个月后，MSCs/CAP复合物移植于颌骨裂区成功地实现了骨再生。破骨细胞能迅速消化CAP颗粒。此外，还实现了正畸牙齿移动到再生骨中。强调MSCs/CAP移植治疗作为一种新的治疗方式具有巨大的潜力，CLP患者的骨缺损，由于颌骨裂骨再生。

The purpose of our study is to establish bone regeneration of jaw cleft by use of bone marrow-derived mesenchymal stem cells (MSCs) with a new scaffold, carbonated-hydroxyapatite (CAP). Artificial jaw cleft was created on the both side of maxilla in Beagle dogs. MSCs were derived from bone marrow of iliac bone by use of an aspiration needle, and cultured. After 1 month, MSCs and new scaffold material, CAP granulated powder, were transplanted into the artificial bone defects. Bone regeneration at jaw cleft site was achieved successfully by the transplantation of MSCs/CAP after 3 months. It is also demonstrated suggested that CAP particles could be digested rapidly by osteoclasts. In addition, orthodontic tooth movement into regenerated bone was also achieved. It is emphasized that MSCs/CAP transplantation therapy has a great potential as a new treatment modality for bone regeneration in CLP patients with bone defect due to jaw cleft.