Interbands: Epigenetic mechanisms of the formation of domains and boundaries on interphase chromosomes

带间：间期染色体上结构域和边界形成的表观遗传机制

• 批准号: 5370890
• 负责人: Professor Dr. Harald Saumweber
• 金额: --
• 依托单位: Arbeitsgruppe Zytogenetik
• 依托单位国家: 德国
• 项目类别: Priority Programmes
• 财政年份: 2002
• 资助国家: 德国
• 起止时间: 2001-12-31 至 2008-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/5370890?language=en
• 关键词: Interbands; Epigenetic; mechanisms; formation; domains

The domain organization of eukaryotic chromatin is an epigenetic phenomenon par excellence. This crucial organization of the interphase chromosome is inherited over cell generations and is seen as a pattern of bands and interbands on polytene chromosomes, which reproducibly form in different tissues and stages of development. The open chromatin of interbands is established by proteins with specific structural and enzymatic activities and is stabilized by elements insulating against influences of nearby condensed chromatin. The aim of the project is to study the mutual interaction of interband specific proteins and their role in establishing open chromatin structure. Their molecular functions in the formation of borders between chromosomal domains and in the formation of insulator elements shall be uncovered. We will establish a model site in the Drosophila genome which allows the exchange of DNA elements by site specific recombination. The role of interband proteins will be studied in the presence of specific mutations, following tissue specific overexpression and induction of RNA interference.

真核细胞染色质的结构域组织是一种典型的表观遗传现象。间期染色体的这种重要组织在细胞世代中遗传，并被视为多线染色体上的带和带间带的模式，其在不同组织和发育阶段中可重复形成。带间的开放染色质由具有特定结构和酶活性的蛋白质建立，并由与附近浓缩染色质的影响绝缘的元件稳定。该项目的目的是研究带间特异性蛋白质的相互作用及其在建立开放染色质结构中的作用。它们在染色体结构域之间的边界形成和绝缘子元件形成中的分子功能将被揭示。我们将在果蝇基因组中建立一个允许通过位点特异性重组交换DNA元件的模型位点。将在存在特定突变的情况下，在组织特异性过表达和诱导RNA干扰后，研究带间蛋白的作用。