Visualization of changes in intracellular ions during glioma cell migration

我们利用细胞内离子成像技术监测了胶质瘤细胞迁移过程中细胞内Ca^<2+>水平和Cl^-的自发变化，并研究了其作用。尽管一些细胞运动明显加速的细胞表现出高频率的自发Ca2+振荡，但细胞内Ca2+水平的变化频率与细胞运动速率无关。我们还通过FRET成像监测了[Cl-]i的时空动态。此外，细胞内Cl-水平的变化可以改变胶质瘤细胞的运动。

We monitored spontaneous changes in intracellular Ca^<2+> level and Cl^- during glioma cell migration using intracellular ion imaging and examined its roles. Although some cells which show marked acceleration of cell motility especially exhibited high frequency of spontaneous Ca2+ oscillation, the frequency of changes in intracellular Ca2+ levels was not correlated with the rate of cell movement. We also monitored the spatio-temporal dynamics of [Cl-]i by FRET imaging. Also, changes in intracellular Cl- levels can modify the cell motility of glioma cells.