The role of ambient GABA in cerebelllar foliation

点击翻译按钮获取中文摘要

Although distribution of GABA-A receptors and GABA-A receptor-mediated responses (excitability) are dynamically changed during stages of cerebellar morphogenesis, its roles on foliation and lobulation of cerebellar cortex have still remained unknown. Here, we addressed this issues by using various imaging methods and in vivo blockade experiments. Optical imaging method of extracellular GABA linked to enzymatic reaction of GABase enabled us to show temporal abundance of ambient GABA in the outer EGL region which have Granule precursor cells (GPCs). We also show that in vivo blockade of GABA-A receptor perturb the proliferation of these GPCs in the the posterior vermis of the early neonatal cerebellum. Furthermore, we have investigated the interaction between GABA-A receptor and sonic hedgehog (SHH) singlings by histochemical and Calcium imaging technique and suggested that SHH signalings seemed not to affect the GABA-A receptor-mediated responses.