Exercise increases neurogenesis around the damaged area after traumatic brain injury

运动可增加脑外伤后受损区域周围的神经发生

• 批准号: 20500472
• 负责人: ITOH Tatsuki
• 金额: $ 3万
• 依托单位: Kinki University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20500472/
• 关键词: 脳外傷; リハビリテーション; 神経幹細胞; 運動; 神経再生; 動動; リハビリ; 記憶改善

Wistar rats received traumatic brain injury (TBI) and were randomly divided into two groups: 1) non-exercise group and 2) exercise group. The exercise group ran on a treadmill for 30 min/day at 22 m/min for 7 consecutive days. Immunohistochemistry was used to monitor neural stem cell (NSC) proliferation around the damaged area and ex vivo techniques were used to isolate NSCs from the damaged region in both groups. The number of nestin as a maker of NSC- and Ki67 as a marker of proliferation cell-positive cells observed at 3 and 7 days after TBI was significantly greater in the exercise group than in the non-exercise group (P<0.01). Furthermore, most nestin-positive cells in the exercise group co-localized with Ki67-positive cells. In ex vivo studies, spheres could be isolated from injured brain tissue from the exercise group at 3 and 7 days following TBI, but at only 3 days in the non-exercise group. The number of spheres isolated from injured brain tissue was greater in the exercise group than in the non-exercise group. Spheres were immunopositive for nestin and comprised of NSCs that could differentiate into neurons and glia. Exercise increases the proliferation of NSCs around the damaged area following TBI. Therefore, exercise therapy (rehabilitation) in the early phase following TBI is important for recuperation from cerebral dysfunction induced by TBI.

将Wistar大鼠随机分为两组：1）非运动组和2）运动组。运动组在跑台上以22 m/min的速度跑30 min/d，连续7 d。免疫组化被用来监测神经干细胞（NSC）周围的受损区域的增殖和离体技术被用来分离神经干细胞从两组的受损区域。在TBI后第3天和第7天观察到的作为NSC标记物的巢蛋白和作为增殖细胞阳性细胞标记物的Ki 67的数量在运动组中显著高于非运动组（P<0.01）。此外，运动组中大多数nestin阳性细胞与Ki 67阳性细胞共定位。在离体研究中，球体可以在TBI后3天和7天从运动组的损伤脑组织中分离出来，但在非运动组中仅在3天。从受伤的脑组织中分离出的球体数量在运动组中比在非运动组中更多。球体呈巢蛋白免疫阳性，由神经干细胞组成，可以分化为神经元和神经胶质细胞。运动增加了创伤后受损区域周围神经干细胞的增殖。因此，TBI后早期的运动治疗（康复）对于TBI所致脑功能障碍的恢复至关重要。

项目成果

Nitorogen-containing bisphosphonate, YM529/ONO-5920, inhibits macrophage inflammatory protein 1α expression and secretion in mouse myeloma cells

含氮双膦酸盐 YM529/ONO-5920 抑制小鼠骨髓瘤细胞中巨噬细胞炎症蛋白 1α 的表达和分泌

• 发表时间: 2008
• 期刊: Cancer Science 99
• 作者: Tsubaki M;et al.
• 通讯作者: et al.

脳外傷損傷局所へのSDF1αとCXCR4アンタゴニスト投与による局所反応の変化に関する免疫組織化学的検討

SDF1α和CXCR4拮抗剂对脑外伤损伤区域局部反应变化的免疫组织化学研究

• 发表时间: 2009
• 作者: Itoh T;et al.
• 通讯作者: et al.

脳卒中易発症性高血圧自然発症ラット(SHESP)大脳における脳卒中発症後の神経幹細胞の出現に関する検討

脑卒中易发性高血压大鼠（SHESP）脑卒中发病后神经干细胞出现的研究

• 发表时间: 2008
• 作者: 伊藤龍生;他
• 通讯作者: 他

Edaravone 投与によるラット脳外傷後の神経幹細胞の出現に関する検討

依达拉奉致大鼠脑损伤后神经干细胞出现的研究

• 发表时间: 2009
• 作者: Itoh T;et al.
• 通讯作者: et al.

Neural stem cell and newly neurons appearance in the cerebral cortex of stroke-prone spontaneously hypertensive rats after stroke (2)

脑卒中后易发生脑卒中的自发性高血压大鼠大脑皮层出现神经干细胞和新神经元 (2)

• 发表时间: 2008
• 作者: Itoh T;et al.
• 通讯作者: et al.