Research on high sensitive handy ELISA system for multi-environmental analysis using 3D micro fluidic networks

酶联免疫吸附试验（ELISA）的实验室-on-a- cd应用受到了广泛关注，因为它便于多个微反应器的自动化[1-2]。然而，由于芯片面积有限，难以同时实现多个反应器单元的性能和集成度。这种局限性带来的最大问题之一是由于固定抗体的表面积不足而导致灵敏度不足。我们提出了一种基于三维（3D）微通道网络的新型cd上实验室概念。本文报道了利用束状毛细管结构在三维实验室光盘上对多氯联苯（PCB）进行高灵敏度检测。

There have been paid much attention to the lab-on-a-CD for the use of enazyme-linked immunosorbent assay (ELISA), due to its convenience for the automation of multiple microreactors [1-2]. However it is difficult to simultaneously realize the performance and integration of multiple reactor units due to the limited chip area. One of the biggest problem arise from the limitation is inadequate sensitivity due to shortage of surface area for the immobilization of antibody. we propose a new type lab-on-a-CD concept based on three-dimensional (3D) microchannel network. This paper reports high-sensitive detection of poly-chlorinatedbiphenyl (PCB) by using bundle-like capillary structure on a 3D lab-on-a-CD.