Research on Full-protocol Disease Marker Screening System using Integrated Lab-on-a-CD for Immunoassay

本文报道了一种具有三维微通道网络和垂直毛细束结构的新型光盘实验室装置。该装置由多个lab-on-a-CD芯片组成，该芯片具有传统的平面微通道和垂直微通道，这些微通道构建在由聚二甲基硅氧烷（PDMS）和聚甲基丙烯酸甲酯（PMMA）制成的磁盘上。采用同步辐射深x射线光刻技术制备了具有垂直通道的PMMA层。我们设计并制作了用于酶联免疫吸附试验（ELISA）的芯片。从计算流体力学的角度提出了Lab-on-a-CD结构的三维液体输运，并成功地在芯片上进行了验证。成功地在三维装置中检测了小鼠免疫球蛋白G(IgG)，值得注意的是，结果显示出相当高的灵敏度，初步结果检测限低至1ng/mL。

This paper reports a new type of lab-on-a-CD device with three-dimensional microchannel networks and vertical capillary bundle structure. The device consist of the multiple lab-on-a-CD chips with conventional planer microchannels and vertical microchannels which constructed on the disks made of poly-dimethylsiloxane (PDMS) and poly-methylmethacrilrate (PMMA). The PMMA layer with vertical channel were fabricated by deep X-ray lithography using synchrotron radiation. We designed and fabricated the chips for aim of taking enzyme-linked immunosorbent assay(ELISA). Three-dimensional liquid transportation through Lab-on-a-CD structure is suggested by computational fluid dynamics and it is successfully demonstrated chips. Detection of immunogroblin G(IgG) from mouse is successfully demonstrated in the three-dimensional devices and it is notable that the result suggests quite high-sensitive implying detection limit down to 1ng/mL by primitive result.