Research on Full-protocol Disease Marker Screening System using Integrated Lab-on-a-CD for Immunoassay

使用集成Lab-on-a-CD进行免疫分析的全方案疾病标志物筛查系统的研究

• 批准号: 23350035
• 负责人: UTSUMI Yuichi
• 金额: $ 11.98万
• 依托单位: University of Hyogo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23350035/
• 关键词: チップ分析; 生体分析; 免疫反応; バイオリアクター; ELISA; マイクロ化学システム; Lab-on-a-chip; Lab-on–a-chip

This paper reports a new type of lab-on-a-CD device with three-dimensional microchannel networks and vertical capillary bundle structure. The device consist of the multiple lab-on-a-CD chips with conventional planer microchannels and vertical microchannels which constructed on the disks made of poly-dimethylsiloxane (PDMS) and poly-methylmethacrilrate (PMMA). The PMMA layer with vertical channel were fabricated by deep X-ray lithography using synchrotron radiation. We designed and fabricated the chips for aim of taking enzyme-linked immunosorbent assay(ELISA). Three-dimensional liquid transportation through Lab-on-a-CD structure is suggested by computational fluid dynamics and it is successfully demonstrated chips. Detection of immunogroblin G(IgG) from mouse is successfully demonstrated in the three-dimensional devices and it is notable that the result suggests quite high-sensitive implying detection limit down to 1ng/mL by primitive result.

本文报道了一种具有三维微通道网络和垂直毛细束结构的新型光盘实验室装置。该装置由多个lab-on-a-CD芯片组成，该芯片具有传统的平面微通道和垂直微通道，这些微通道构建在由聚二甲基硅氧烷（PDMS）和聚甲基丙烯酸甲酯（PMMA）制成的磁盘上。采用同步辐射深x射线光刻技术制备了具有垂直通道的PMMA层。我们设计并制作了用于酶联免疫吸附试验（ELISA）的芯片。从计算流体力学的角度提出了Lab-on-a-CD结构的三维液体输运，并成功地在芯片上进行了验证。成功地在三维装置中检测了小鼠免疫球蛋白G(IgG)，值得注意的是，结果显示出相当高的灵敏度，初步结果检测限低至1ng/mL。

项目成果

Contamination-Free Internally-Triggered Automatic Flow Sequencing for Microfluidics

适用于微流体的无污染内部触发自动流程排序

• 发表时间: 2012
• 作者: Masaki Ishizawa;Yuichi Utsumi
• 通讯作者: Yuichi Utsumi

Three Dimensional Gold Nanostructure for Plasmonic Sensing

用于等离子体传感的三维金纳米结构

• 发表时间: 2012
• 作者: Ryo Takahashi;Takao Fukuoka;Yuichi Utsumi
• 通讯作者: Yuichi Utsumi

Three-Dimensional Magneto Impedance Sensor using a Multi Layers Electroplating technique

采用多层电镀技术的三维磁阻抗传感器

• 发表时间: 2013
• 作者: Yuji Yoritama;S. Yamamoto;T. Uchiyama ;Akinobu Yamaguchi;Yuichi Utsumi
• 通讯作者: Yuichi Utsumi

A Chip-Size Structure for Continuous Microwave Irradiation by Post-Wall Waveguide

一种用于壁后波导连续微波照射的芯片尺寸结构

• 发表时间: 2012
• 作者: Mitsuyoshi Kishihara;M. Yamashima;Yuichi Utsumi
• 通讯作者: Yuichi Utsumi

Development of Bio-Microsystems and Sensors using nano/micro-structures

使用纳米/微米结构开发生物微系统和传感器

• 发表时间: 2012
• 作者: 山崎悟;坂部輝御;長谷川健;泰永愛佳;Yuichi Utsumi
• 通讯作者: Yuichi Utsumi