OMICS ANALYSIS TO INVESTIGATE ROLES OF MICRORNAS IN CANCER

通过组学分析研究微核在癌症中的作用

• 批准号: 20510184
• 负责人: FUKUOKA Yutaka
• 金额: $ 2.91万
• 依托单位: Tokyo Medical and Dental University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20510184/
• 关键词: システムゲノム; がん; マイクロRNA; 遺伝子; 発現; マイクロアレイ; microRNA; バイオインフォマティクス; 肝臓がん; 遺伝子発現; 肝細胞がん

To investigate the local effect of miRNA in cancer, we analyzed gene and miRNA expressions in hepatocellular carcinoma (HCC) and surrounding non-tumor tissues. We calculated the Pearson correlation coefficients between miRNAs neighboring genes and compared the coefficients in the tumor and non-tumor tissues. This analysis was repeated for intronic and intergenic miRNAs. Finally, the correlation coefficients between miRNAs and their target genes were compared in the tumor and non-tumor tissues. The results suggested that in HCC, more miRNAs correlated with neighboring genes positively and that the correlation coefficients between the intronic miRNAs and their host genes were higher in HCC. The correlation analysis between miRNAs and their targets suggested that there was no significant difference between the correlation coefficients in the tumor and non-tumor tissues.

为了研究miRNA在癌症中的局部作用，我们分析了肝细胞癌（HCC）和周围非肿瘤组织中的基因和miRNA表达。我们计算了miRNA相邻基因之间的皮尔逊相关系数，并比较了肿瘤和非肿瘤组织中的系数。对于内含子和基因间miRNA，重复了此分析。最后，在肿瘤和非肿瘤组织中比较了miRNA及其靶基因之间的相关系数。结果表明，在HCC中，更多的miRNA与邻近基因相关，并且内含子miRNA及其宿主基因之间的相关系数在HCC中更高。 miRNA及其靶标之间的相关分析表明，肿瘤和非肿瘤组织的相关系数之间没有显着差异。

项目成果

MUC12 mRNA expression is an independent marker of prognosis in stage II and stage III colorectal cancer

• DOI: 10.1002/ijc.25256
• 发表时间: 2010-11-15
• 期刊: INTERNATIONAL JOURNAL OF CANCER
• 影响因子: 6.4
• 作者: Matsuyama, Takatoshi;Ishikawa, Toshiaki;Sugihara, Kenichi
• 通讯作者: Sugihara, Kenichi

Genome-wide DNA methylation analysis in tumor and normal tissues from lung squamous cell carcinoma and serous cystadenocarcinoma

肺鳞状细胞癌和浆液性囊腺癌肿瘤和正常组织的全基因组 DNA 甲基化分析

• 发表时间: 2010
• 作者: 稲岡秀検;福岡豊;野城真理
• 通讯作者: 野城真理

肝細胞がんにおけるmicroRNA発現量と近傍遺伝子発現量の関係

肝细胞癌中microRNA表达量与邻近基因表达量的关系

• 发表时间: 2009
• 作者: 岩村泰輔;福岡豊;内山孝憲;稲岡秀検;Mahmut Y;水島洋;田中真二;有井滋樹;野城真理;田中博;Kohane IS
• 通讯作者: Kohane IS

Clinical gene network analysis on inflammatory bowel disease

炎症性肠病临床基因网络分析

• 发表时间: 2010
• 作者: Suzuki S;Takai-Igarashi T;Fukuoka Y;Tanaka H;Fusaro V;Pivovarov R;Tonellato PJ
• 通讯作者: Tonellato PJ

Microarray analysis of mRNA expressions related to IL-6 in stretched normal human pulmonary artery endothelial cells

正常人肺动脉内皮细胞拉伸后IL-6相关mRNA表达的微阵列分析

• 发表时间: 2010
• 作者: 小林こず恵;田中真澄;稲岡秀検;根武谷吾;福岡豊;小林弘祐;野城真理
• 通讯作者: 野城真理