The molecular conformation of amyloid fibril revealed by pressure axis

压力轴揭示淀粉样原纤维的分子构象

• 批准号: 20550025
• 负责人: TANIGUCHI Yoshihiro
• 金额: $ 3.08万
• 依托单位: Ritsumeikan University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20550025/
• 关键词: 圧力; インシュリン; アミロイド線維; 二次構造; 分子構造; FT-IR; NMR; インスリン; 変性中間体; 温度; 高エネルギー構造; PLGA; FTIR分光法; 活性化体積

The pressure-induced changes in the secondary structure of insulin amyloid fibril up to 1100MPa were studied by means of the high pressure Fourier transform infrared spectroscopy. The early amyloid fibril to come from intermediate state of insulin was melted by high compression up to 550MPa. But the aged amyloid fibril does not change to be rigid structure by very high compression up to 1GPa.The detail structure of insulin under high pressure was studied by high pressure one- and two-dimensional NMR spectroscopy. High pressure NMR showed large changes in amide proton chemical shift for the residues mainly in the N- and C-terminal strands of B chain and helix1 of A-chain. The structural changes in the N- and C-terminal strands of B-chain under high pressure is a key factor for the pressure induced molecular association and aggregation of insulin

用高压傅里叶变换红外光谱研究了压力对胰岛素淀粉样原纤维二级结构的影响。早期的淀粉样原纤维来自胰岛素的中间状态，在高达550 Mpa的高压下被融化。通过高压一维和二维核磁共振波谱研究了高压下胰岛素的详细结构。高压核磁共振显示，残基的酰胺质子化学位移变化很大，主要在B链的N-末端和C-末端以及A-链的Helix1。高压下B链N-端和C-端链的结构变化是压力诱导胰岛素分子缔合和聚集的关键因素

项目成果

The secondary struct ure of pressure- and heat-induced protein aggregates and amyloid fibrils studied by high pressure FTIR spectroscopy

高压 FTIR 光谱研究压力和热诱导的蛋白质聚集体和淀粉样原纤维的二级结构

• 发表时间: 2008
• 作者: Y.Taniguchi;A.Okuno;R.Yokoyama;M.Kato
• 通讯作者: M.Kato

Pressure-induced helix-coil transition of poly-L-gultamic acid in water

聚-L-谷氨酸在水中压力诱导的螺旋-螺旋转变

• 发表时间: 2008
• 期刊: J.Physics Conference Series 121
• 作者: T.Takekiyo;Y.Yoshimura;A.Okuno;A.Shimizu;M.Kato;Y.Taniguchi
• 通讯作者: Y.Taniguchi

Pressure effects on the structure, kinetic, and thermodynamic properties of heat-induced aggregation of protein studied by FT-IR spectroscopy

通过 FT-IR 光谱研究压力对热诱导蛋白质聚集的结构、动力学和热力学性质的影响

• DOI: 10.1088/1742-6596/215/1/012156
• 发表时间: 2010
• 作者: Y. Taniguchi;A. Okuno;M. Kato
• 通讯作者: M. Kato

High Pressure NMR reveals a locally disordered conformation foinsulin

高压核磁共振揭示了胰岛素局部无序构象

• 发表时间: 2010
• 作者: R.Kitahara;A.Tanji;S.Kitazawa;Y.Taniguchi
• 通讯作者: Y.Taniguchi

Uncovering the physical origin of the difference between aliphatic chain and aromatic ring in the "hydrophobic" effect on partial molar volume

揭示脂肪链和芳香环对偏摩尔体积的“疏水”效应差异的物理根源

• 发表时间: 2008
• 期刊: J.Chem.Phys. 128
• 作者: T.Imai;Y.Hisadomi;S.Sawamura;Y.Taniguchi
• 通讯作者: Y.Taniguchi