通電式計測システムによる微量重金属イオンの特異的分析

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A flow-injection microdetermination system armed with an electrolytic device for trace amount of heavy metal ions was developed. A unique analytical method based on the apoenzyme activation originally created by the author was applied to realize microanalysis of heavy metal ions with use of enzymatic methods. Use of applications of 2 mA direct current across two tubular platinum electrodes set between an inlet and also outlet of the enzyme column, and also of which pH controlled at the isoelectric point, showed that removal of heavy metal ions coordinated in the active site of the enzymes was positively done and then, the enzymes could be converted to the corresponding apoenzymes. Fabrication of a flow-through reactor armed with an electrolytic device in combination with a planar type of immobilized enzymes, suffering from no increase in terminal voltage due to application of direct currents, could be successfully realized.