The evaluation of plasma-membrane injury and the effect of a moisturizer on injury reduction in freezing of tissue level

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In this study, influence of the contact state of cells in the sample of tissue level as high cell density on a plasma-membrane injury in a freeze-thaw process was investigated using the monolayer-culture cell which controlled the orientation and the cell density. Furthermore, feasibility of the improvement in cell viability by the hydrate near the plasma membrane of the hyaluronan which is a moisturizer was investigated. As a result, it was suggested that a plasma-membrane injury increased with increasing the cell density, and that the extent of an injury depended on a cell orientation and freezing sequences. Moreover, it is clear that hyaluronan has cryoprotective effect for plasma-membrane.