The evaluation of plasma-membrane injury and the effect of a moisturizer on injury reduction in freezing of tissue level

组织冷冻水平下质膜损伤的评价和保湿剂对减轻损伤的影响

• 批准号: 20560197
• 负责人: UJIHIRA Masanobu
• 金额: $ 3万
• 依托单位: Kitasato University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20560197/
• 关键词: 生体熱工学; 生体組織の凍結保存; ヒアルロン酸; 凍結保護効果; 単層培養細胞; 細胞の配向制御; ヒト皮膚繊維芽細胞; ヒアルロン酸ナトリウム; 凍結方向; 蛍光染色

In this study, influence of the contact state of cells in the sample of tissue level as high cell density on a plasma-membrane injury in a freeze-thaw process was investigated using the monolayer-culture cell which controlled the orientation and the cell density. Furthermore, feasibility of the improvement in cell viability by the hydrate near the plasma membrane of the hyaluronan which is a moisturizer was investigated. As a result, it was suggested that a plasma-membrane injury increased with increasing the cell density, and that the extent of an injury depended on a cell orientation and freezing sequences. Moreover, it is clear that hyaluronan has cryoprotective effect for plasma-membrane.

在这项研究中，细胞的接触状态的影响，在组织水平的样品作为高细胞密度的质膜损伤在冻融过程中进行了研究，使用单层培养细胞，控制的方向和细胞密度。此外，研究了通过作为保湿剂的透明质酸的质膜附近的水合物改善细胞活力的可行性。结果表明，质膜损伤随着细胞密度的增加而增加，损伤的程度取决于细胞取向和冷冻顺序。透明质酸对质膜有明显的低温保护作用。

项目成果

Observation of Survival Distribution in Monolayer Cells after Cryopreservation : Influence of Cell Density on Post-thaw Viability

冷冻保存后单层细胞存活分布的观察：细胞密度对解冻后活力的影响

• 发表时间: 2009
• 作者: Akira Iwama;Ken-ichiro Shibuya;Aya Matsui;Masanobu Ujihira
• 通讯作者: Masanobu Ujihira

Cryoprotective Effect of Low-Molecular-Weight Hyaluronan on Human Dermal Fibroblast Monolayers

低分子量透明质酸对人真皮成纤维细胞单层的冷冻保护作用

• 发表时间: 2010
• 期刊: CryoLetters Vol.31 No.2
• 作者: Masanobu Ujihira;Akira Iwama;Makie Aoki;Kanako Aoki;Sayaka Omaki;Erika Goto;Kiyoshi Mabuchi
• 通讯作者: Kiyoshi Mabuchi

The effect of Cell Orientation Control on the Cell Viability after Cryopreservation in Monolayer Cells Cultured by Arbitrary Cell Densities

任意细胞密度单层细胞冻存后细胞方向控制对细胞活力的影响

• 发表时间: 2010
• 作者: Akira Iwama;Masanobu Ujihira;Yasuhiro Saiki
• 通讯作者: Yasuhiro Saiki

厚みを持つ組織の凍結保存における解凍後生存率の改善に関する検討

提高厚组织冻存解冻存活率的研究

• 发表时间: 2010
• 作者: 岩間輝;大浦和宏;氏平政伸
• 通讯作者: 氏平政伸

Investigation of the cryoprotective effect of low-molecular-weight hyaluronic acid on human dermal fibroblast monolayers

低分子透明质酸对人真皮成纤维细胞单层冷冻保护作用的研究

• 发表时间: 2009
• 作者: 岡本達幸;名塩博史;Masanobu Ujihira
• 通讯作者: Masanobu Ujihira