The improvement effect of xenon gas on cellular viability for cryopreservation of biological tissue

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The purpose of this task was to clarify the efficiency of a xenon gas in order to reduce the damage by the intercellular stress during freezing and the cellular damage by the low temperature itself under an unfrozen condition in cryopreservation of a sample with a level of thick biological tissue. First, the enhanced effect of reduction of cellular damage in freezing process of a cell with a combination of dimethylsulfoxide as a typical cryoprotectant and pressurized dissolution and decompression of xenon gas was examined. And then the protective effect to the cell against the stress of the low temperature itself at 4 degree Celsius was examined. As a result, when a sample was frozen at a low cooling rate, it was suggested that dissolution of xenon gas was effective in reduction of damage by the intercellular stress, and it was clear that pressurized addition of xenon gas was effective in reduction of the cellular damage by the low temperature itself under an unfrozen condition.