The improvement effect of xenon gas on cellular viability for cryopreservation of biological tissue

氙气对生物组织冻存细胞活力的改善作用

• 批准号: 23560241
• 负责人: UJIHIRA Masanobu
• 金额: $ 3.41万
• 依托单位: Kitasato University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23560241/
• 关键词: 低温保存; 凍結保存; キセノンガス; 細胞障害; 保護効果; 細胞損傷; ヒト皮膚繊維芽細胞

The purpose of this task was to clarify the efficiency of a xenon gas in order to reduce the damage by the intercellular stress during freezing and the cellular damage by the low temperature itself under an unfrozen condition in cryopreservation of a sample with a level of thick biological tissue. First, the enhanced effect of reduction of cellular damage in freezing process of a cell with a combination of dimethylsulfoxide as a typical cryoprotectant and pressurized dissolution and decompression of xenon gas was examined. And then the protective effect to the cell against the stress of the low temperature itself at 4 degree Celsius was examined. As a result, when a sample was frozen at a low cooling rate, it was suggested that dissolution of xenon gas was effective in reduction of damage by the intercellular stress, and it was clear that pressurized addition of xenon gas was effective in reduction of the cellular damage by the low temperature itself under an unfrozen condition.

本任务的目的是阐明氙气的效率，以减少冷冻过程中细胞间应力造成的损伤以及在未冷冻条件下低温本身造成的细胞损伤，以冷冻保存具有一定厚度的生物组织的样品。首先，检查了作为典型冷冻保护剂的二甲基亚砜与氙气的加压溶解和减压的组合在细胞冷冻过程中减少细胞损伤的增强效果。然后考察了细胞对4摄氏度低温本身应激的保护作用。结果表明，当以低冷却速率冷冻样品时，氙气的溶解对于减少细胞间应力造成的损伤是有效的，并且清楚的是，加压添加氙气对于减少非冷冻条件下低温本身造成的细胞损伤是有效的。

项目成果

Xeガスによる人皮膚線維芽細胞の低温障害に対する保護効果

氙气对人体皮肤成纤维细胞低温损伤的保护作用

• 发表时间: 2011
• 作者: Tomohiko Yamaguchi;Qian Wang;Yuying Yan;岩間輝,川口翔子,氏平政伸
• 通讯作者: 岩間輝,川口翔子,氏平政伸

細胞の凍結保存におけるキセノンガス加圧添加による保護効果

加压氙气对细胞冷冻保存的保护作用

• 发表时间: 2013
• 作者: Yamaguchi;T.;Yan;Y.;青野佳菜子,佐藤航,氏平政伸
• 通讯作者: 青野佳菜子,佐藤航,氏平政伸

Protective Effects of Pressurized Addition of Xenon Gas against Cold Damage in Cell Monolayer

加压添加氙气对单层电池冷损伤的保护作用

• 发表时间: 2013
• 作者: Seino K;Sato K;Ujihira M.
• 通讯作者: Ujihira M.

細胞の凍結保存におけるキセノンガスの保護効果

氙气对细胞冻存的保护作用

• 发表时间: 2012
• 作者: S. Momoki;T. Fukunaga;K. Narumiya;T. Arima;T. Yamaguchi;T. Shigechi;青野佳菜子,氏平政伸
• 通讯作者: 青野佳菜子,氏平政伸

キセノンガスの加圧添加による細胞の低温障害の低減

通过加压添加氙气减少低温对电池的损伤

• 发表时间: 2012
• 作者: Yamaguchi;T. and Yan;Y.Y.;畑 幸一，福田 勝哉，増崎 貴，羽村 光道;青野佳菜子,氏平政伸
• 通讯作者: 青野佳菜子,氏平政伸