The improvement effect of xenon gas on cellular viability for cryopreservation of biological tissue

这项任务的目的是阐明氙气的效率，以减少细胞间应力在冷冻期间的损伤和低温本身在非冷冻条件下对具有一定厚度的生物组织样本的低温保存。首先，研究了二甲基亚砜作为典型的冷冻保护剂与氙气加压溶解减压的组合对细胞冷冻过程中细胞损伤的增强作用。然后研究了细胞在4摄氏度低温下对自身压力的保护作用。结果表明，当样品以低冷却速率冷冻时，氙气的溶解可以有效地减少细胞间应力造成的损伤，而在非冷冻条件下，加压添加氙气可以有效地减少低温本身造成的细胞损伤。

The purpose of this task was to clarify the efficiency of a xenon gas in order to reduce the damage by the intercellular stress during freezing and the cellular damage by the low temperature itself under an unfrozen condition in cryopreservation of a sample with a level of thick biological tissue. First, the enhanced effect of reduction of cellular damage in freezing process of a cell with a combination of dimethylsulfoxide as a typical cryoprotectant and pressurized dissolution and decompression of xenon gas was examined. And then the protective effect to the cell against the stress of the low temperature itself at 4 degree Celsius was examined. As a result, when a sample was frozen at a low cooling rate, it was suggested that dissolution of xenon gas was effective in reduction of damage by the intercellular stress, and it was clear that pressurized addition of xenon gas was effective in reduction of the cellular damage by the low temperature itself under an unfrozen condition.