Identification of novel ghrelin receptor in fish and clarification of the relationship between the receptor and biological action.

鱼类中新型生长素释放肽受体的鉴定并阐明受体与生物作用之间的关系。

• 批准号: 20570065
• 负责人: KAIYA Hiroyuki
• 金额: $ 2.83万
• 依托单位: National Cardiovascular Center Research Institute
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20570065/
• 关键词: グレリン; グレリン受容体; 魚類; キンギョ; 受容体; 脳; 腸; 内在性; ペプチド; 精製; GHS-R1a; GHS-R2a; 変異体; ゲノム遺伝子; 組織分布; cDNA

In this study, we cloned cDNAs for four functional ghrelin receptors (GHS-R1a-1, 1a-2, 2a-1 and 2a-2) that composed of 360, 360, 366 and 367 amino acids, respectively in goldfish brain. Each receptor mRNA showed tissue-specific expression, suggesting that each receptor mediates tissue-specific action of ghrelin in goldfish. One species of ghrelin receptor, GHS-R1a has been known to be present in vertebrates other than fish examined so far. We tried to isolate new ligands for the new functional GHS-Ra, which is the novel type of ghrelin receptor, from tissue extracts of goldfish brain and intestine, but unfortunately the new ligand was not able to find out during the course of this study.

本研究从金鱼脑中克隆了四种具有生长激素释放肽功能的受体（GHS-R1 a-1、1a-2、2a-1和2a-2）的cDNA，它们分别由360、360、366和367个氨基酸组成。每个受体的mRNA表现出组织特异性表达，表明每个受体介导的组织特异性作用的生长激素释放肽在金鱼。生长激素释放肽受体GHS-R1 a是目前已知的存在于脊椎动物中的一种受体，而不是鱼类。我们试图从金鱼脑和肠的组织提取物中分离新的功能性GHS-Ra的新配体，GHS-Ra是一种新型的生长激素释放肽受体，但不幸的是，在本研究过程中未能发现新的配体。

项目成果

Ghrelin modulates fatty acid synthase and related transcription factor mRNA levels in a tissue-specific manner in neonatal broiler chicks

• DOI: 10.1016/j.peptides.2009.04.015
• 发表时间: 2009-07-01
• 期刊: PEPTIDES
• 影响因子: 3
• 作者: Buyse, Johan;Janssen, Sara;Dridi, Sami
• 通讯作者: Dridi, Sami

Identification and genomic sequence of a ghrelin receptor (GHS-R)-like receptor in the Mozambique tilapia, Oreochromis mossambicus.

莫桑比克罗非鱼 Oreochromis mossambicus 中生长素释放肽受体 (GHS-R) 样受体的鉴定和基因组序列。

• 发表时间: 2009
• 期刊: Zoolog Sci. 26(5)
• 作者: Kaiya H;Riley LG;Janzen W;Hirano T;Grau EG;Miyazato M;Kangawa K.
• 通讯作者: Kangawa K.

Absence of effects of short-term fasting on plasma ghrelin and brain expression of ghrelin receptors in the tilapia, Oreochromis mossambicus.

短期禁食对罗非鱼（Oreochromis mossambicus）血浆生长素释放肽和脑生长素释放肽受体的表达没有影响。

• 发表时间: 2008
• 期刊: Zoolog Sci 25(8)
• 作者: Riley LG;Fox BK;Breves JP;Kaiya H;Dorough CP;Hirano T;Grau EG.
• 通讯作者: Grau EG.

Purification and properties of ghrelin from the intestine of the goldfish, Carassius auratus

• DOI: 10.1016/j.peptides.2008.12.016
• 发表时间: 2009-04-01
• 期刊: PEPTIDES
• 影响因子: 3
• 作者: Miura, Tohru;Maruyama, Keisuke;Matsuda, Kouhei
• 通讯作者: Matsuda, Kouhei

Ghrelin-like peptide with fatty acid modification and O-glycosylation in the red stingray, Dasyatis akajei.

• DOI: 10.1186/1471-2091-10-30
• 发表时间: 2009-12-14
• 期刊: BMC biochemistry
• 作者: Kaiya H;Kodama S;Ishiguro K;Matsuda K;Uchiyama M;Miyazato M;Kangawa K
• 通讯作者: Kangawa K