Analysis of Molecular Mechanism for Regulating Initiation of Recombination-mediated Mitochondrial DNA Replication

重组介导的线粒体DNA复制调控起始的分子机制分析

• 批准号: 20570171
• 负责人: LING Feng
• 金额: $ 3.16万
• 依托单位: The Institute of Physical and Chemical Research
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20570171/
• 关键词: 遺伝学; 遺伝子; 核酸; ストレス; 酵素; DNAの組換え; ミトコンドリア; Saccharomyces cerevisiae; Saccharomvces cerevisias; 組換え; 相同DNA対合; 除去修復; ローリングサークル型複製; 酸化ストレス応答; DNA複製

The mechanism for initiation of recombination-mediated mitochondrial DNA (mtDNA) replication is still unclear in budding yeast. We revealed that the oxidative DNA damage-excision repair enzyme Ntg1 recognizes the exposed single-strand region at the sites of oxidative modifications and introduces DNA double-stranded breaks at a replication origin. In addition, the 5'-3' exonuclease and DNA helicases play roles in mtDNA replication-mediated by Mhr1, which in vitro prefers relaxed closed circular double-stranded DNA for catalyzing homologous DNA pairing.

重组介导的线粒体DNA（mtDNA）复制的启动机制仍不清楚。我们透露，氧化DNA损伤拆卸酶NTG1在氧化修饰部位识别裸露的单链区域，并在复制起源引入了DNA双链断裂。此外，5'-3'外丝酶和DNA解旋酶在由MHR1介导的mtDNA复制中起着作用，MHR1在体外更喜欢放松的闭合闭合圆形双链DNA，用于催化同源DNA配对。

项目成果

The yeast checkpoint kinase Dun1 down-regulates DIN7 in the absence of DNA damage

在没有 DNA 损伤的情况下，酵母检查点激酶 Dun1 下调 DIN7

• 发表时间: 2008
• 期刊: Bioscience, Biotechnology, and Biochemistry 72(6)
• 作者: Ayako Yoshitani;Feng Ling;Minoru Yoshida
• 通讯作者: Minoru Yoshida

ROSで誘導される組換え依存型ミトコンドリアDNA複製

ROS诱导的重组依赖性线粒体DNA复制

• 发表时间: 2009
• 作者: 凌楓;堀晶子,牛栄;柴田武彦;吉田稔
• 通讯作者: 吉田稔

DNAの位相幾何学的変化を伴わない組換え中間体形成

DNA 中无拓扑变化的重组中间体形成

• 发表时间: 2009
• 作者: 堀晶子;吉田稔;凌楓;堀晶子;柴田武彦;柴田武彦
• 通讯作者: 柴田武彦

Heteroduplex Joint Formation Free of Net Topological Change by Mhr1, a Mitochondrial Recombinase

• DOI: 10.1074/jbc.m900023200
• 发表时间: 2009-04-03
• 期刊: JOURNAL OF BIOLOGICAL CHEMISTRY
• 影响因子: 4.8
• 作者: Ling, Feng;Yoshida, Minoru;Shibata, Takehiko
• 通讯作者: Shibata, Takehiko

Mitochondrial fusion increases mitochondrial DNA copy number in budding yeast

线粒体融合增加芽殖酵母中线粒体 DNA 拷贝数

• 发表时间: 2011
• 期刊: Genes to Cells
• 影响因子: 2.1
• 作者: Feng Ling;Tsutomu Mikawa;Takehiko Shibata;Akiko Hori
• 通讯作者: Akiko Hori